The principle of Real Time PCR, Reverse Transcription, quantitative rt-PCR

This video is an easy and full explanation about the principle of real time PCR.
For better understanding watch the previous video about the principle of PCR:

[sherylrodriguez]

Good video! just a quick comment to clarify that RT-PCR is Reverse Transcription PCR and Real Time PCR is Q-PCR, for Quantitative PCR, to avoid confusion. Thanks for your video! It was helpful

[manuelmendez]

I came here crying due to I couldn't understand my class today. I left loving RT PCR. Amazing work! Thank you so so muuuuch!

[reindorfboateng]

This 5 years ago video has provided me with great knowledge and insight. Thank you very much for the clear and detailed explanation

[antonioassis]

Seriously, your video is totally amazing. What you have shown here, I guarantee you, most people from the field doesn't know. Just one little insignificant correction, cDNA means "complementary DNA" and don't "circle DNA".

[yashkarmarkar]

This is the most logical progression for someone who's looking to understand PCR. Really well structured content, big up!

[farshidyekani]

Good presentation .. Just an important point: cDNA is Not cyclic DNA, It's Called Complementary DNA.

[Tony_Ss-bxdp]

It is an amazing video! thanks a lot for this explanation. Just one correction; cDNA is for "complementary" not for "cyclic" (22:04). Great job!

[dryounisrather]

i am not a molecular biologist but i can follow and understand your lecture easily, now i can interpret the COVID19 RT-PCR diagnostic result just looking at the curves and figuring out CT value quite easily, thanks and keep sharing knowledge

[DM-xqqg]

your channel deserves more views! i could not have done well at university without you

[abhayiyer]

U explain well. I didn't understand RT PCR for my course. Now I understand it. U have great teaching talent.

[austinmiles]

This is absolutely incredible. Just what I needed for a presentation next week.

[cyrtcheu]

This video is very good, very absorbing, you explained this methods simply and precisely. The only thing I can say is that SYBR green instead of Cyber green. It's very interesting.

[danial]

I have to admit, pretty impressive ability to comunicate, you really made it cristal clear despite english not being your first language. Thank you very much!

[munerhd]

Omg thank you much so much 😭 do you know how many times i tried to understand and looking for videos seeking god to understand this pain in the neck machine?!!! You’re awesome I finally understand it again thank you so much my savior 🥺💕

[selinunsalserbes]

A correction: the reason of becoming non-exponential phase means there is no more reaction components anymore ( dna pol, dntps etc. run out). The explanation of becoming non-exponential phase is not dna polimerase becomes inactive. By the way, that is a great video, thank you!

[adlesal]

You are magnificent.
You do not leave any point without detailed explanation.
God blessings ❤

[nandinipriyathangavel]

Thank you madam for your awesome explanation....first I didn't understand the rt PCR before now I'm clear in this topic ...once again thank you madam

[Zweileiter]

great video! I already have a Master in Biologie, but never was interested so much in molecular biologie and I totaly get everything about qPCR now! Thanks!!!

[tianecheverry]

A really helpful video but I think that it is SYBR Green not Cyber green, it really helps me to clarify some doubts that I had thank you so much, by the last qPCR: IS REAL-TIME PCR and RT-PCR: is REVERSE TRANSCRIPTION PCR.

[ilaydademir]

all mechanisms explained clearly! no insignificant details, just the main points, thank you : )