SYBR Green qPCR

SYBR Green qPCR allows monitoring of amplification of any double-stranded DNA sequence. It does not require probes and thus reduces assay setup and running costs. Also, multiple dyes can bind to a single amplified molecule, which increases sensitivity for detecting amplification products.

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[matias]

Tomorrow I have a job interview, and this video helped me remember many aspects of PCR that I had forgotten, I am very grateful!

[연만-bm]

It was really useful to understand the principle of sybr green!!😊

[cristv.a.]

what a beautiful video, I love the explanation <3

[makendratherealityseeker]

Does anybody answer what is the full form of SYBR?

[itsmejulia]

Would this technology be useful for determining viral load? For example if someone is infected with a DNA virus, would this be able to determine how much of the virus the patient has in his/her blood?

[orion]

Will it lead to false positives? Like the sybr green can intetcalate into the primer dimer and may give false positive results?

[weimullerjohann]

You can cut up many spaghetti and then multiply them unknown. Cheeky rip off nonsense.