qPCR (real-time PCR) protocol explained

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Hey Friends,

qPCR is frequently used in many labs around the world. It makes up for the major part of the testing strategy against Covid-19 right now and is applied whenever one has to determine gene expression analysis. Real-time PCR works with fluorescent reporters so that the reaction progression is visualized after each cycle.

Cheers
Henrik

Time Codes:
0:00 quantitative PCR
2:15 SYBR Green qPCR
2:42 TaqMan Probe qPCR
3:31 qPCR results
4:14 Applications
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Bro you just saved me from pulling an all nighter for my molecular biology homework thx a lot

juliotironiferreiradacosta
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That was very well explained. I had a hard time understanding this mechanism in other videos, but yours just made it so easy. Thank you for sharing!!

berelhumain.e
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This video absolutely made me understand within 4 min, thanks a lot for giving the light to the novel one

Menanonyung
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i've not watched an explanation simpler, clearer and detailed than this, thanks buddy!

mr.awesomevibes
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This is by far the best qPCR video I've seen and I have been searching for a solid 3-4 hours.
You're my hero, thank you.

seniorgir
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Thanks for this! Super easy to understand. I'm using qPCR next week for my undergrad research.

erinsloan
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Excellent summary on the mechanism of Taq Man's work. Cleared the whole concept for me there!

RohitPant
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I love how short and to the point your videos are. Great job!

adser
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This video made me understand what is qPCR. thanks for making an easy video that can be viewed by science students. more blessing

habimanaarsene
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OMGG, thank you so much, explained very nicely & in a simple fashion 👍🏻

harshchaturvedi
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Love the way you explain things! So clear and easy to understand!

meowmeow
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Oh god that was quick and effective. Unbelievable. Thank you

emirkaplan
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I love videos that explain a video well and in a few minutes time, thank you!

madisonmoore
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reallygreat video again. Me and my friend are having such an easy time understanding this content.

dobby
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Love the German accent and your explanations, keep going!

marekintal
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Great video!!! The pronunciation, the video, and the content, all of these are very valuable! Could you please produce a video on how does loop-mediated isothermal amplification works? Thanks in advance! you are such a great Toutuber!

qiaofengli
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Thank you so much, Perfectly summarized. You earned yourself a subscriber😊.

sarnaver
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this explanation is clear and really helpfull. Thank you so much. love this channel

qshjxhw
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just found the perfect explanation! thank you very much from Spain :)

GabrielaFranque
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i was reading the wiki article about this one just now, what a coincidence!

thank you!

PunitorMaximus