Reverse Transcriptase PCR (RT PCR) | What are the applications of RT PCR ? | RT PCR protocol

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This video talks about the principle behind RT PCR. Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR).[1] It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Combined RT-PCR and qPCR are routinely used for analysis of gene expression and quantification of viral RNA in research and clinical settings.

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Sorry, I can't give a 'super thanks', but I would like to express my sincere thanks for your videos. I am able to understand a lot about biotechnology and then passed my exam. I'm a vet student from Indonesia. 🙏

florenciachandrikahalim

Thank you so much for these very useful short tips!

alejandropikoulasplata

Hello Bro
You have not included the further steps
Like After making the hybrid rna-dna .The hybrid's rna is partially digest by Rnase H, then DNA polymerase I forms a complimentary strand and nick is sealed by DNA ligase and
Thus forms a cDNA

IronMan-djuw

very good, detailed and concise explaination, thank you sir!

davidace

Very well explained thanks !! The animations are really good

jagjyotkaur

Very good info! Do you have the references??

mariafernandalealtamez

Please upload a video on allele specific PCR.

anusreesaha

I can't thank u enough for this... just keep going 🙏🙏🙏

priyankachauhan

Great video, comparison of RT-PCR and PCR was a benefit

ikennafranklinokoro

Amazing explanation ... thank u sir...🙏🙏🙏🤗🤗🤗🤗🤗🤗

geekymedico.

Sir is this technique used for hiv detection

sanjeevyadav

Thanks, good content, should give you thumb up.

pengxiong-wu

I have a question: when I have transcribed the mRNA into cDNA, do I perform a normal PCR with the cDNA?
Can someone pls tell me if i'm right or wrong ?

PCR steps
1) denaturation of the template DNA
2) use primer and poymerase
3) amplification

RT-PCR:
1) isolation of RNA
2) transcribe with reverse primer and enzyme reverse transcriptase into cDNA
3) follow PCR steps

SuperLinhi

you saved my todays prentation sir, thank you very much!

nilufertek

very nice channel thanks from turkey arpan

alperentur

Yawrrr such a super duper lecture thank you so much🥹✌️

sanajiya

Hi thank You for the well explained video can you explain microRNAs

Yousha

sir is rt pcr is same technique used in covid rest or it is different .

shubhamrana

Dont we use a specific primer in RT pcr with those 2 primers?

__jm__

Hello sir, i want to go for research but i don't know how to find research institute for my topic of research can u help me that how can i search institute.

ritikyadav

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