Quantitative real time PCR (qPCR)

[morvolio-]

This is that guy on youtube that explains ambiguous concepts very well, which you are searching for. Amazing!

[emmaadams]

Using this to prep for a mock interview tomorrow. You're a lifesaver. Thank you!

[rosegott]

Really well done, thanks! I wish someone had walked me through qPCR this well when I first started running them!

[rutovincentk]

This is the best explanation, I have ever seen I can now understand Real time qPCR

[kavitharachaiah]

Clear explanation. But do add some more text in your slides, like principle of the technique & lable each & every component in the slides. So that it will be much useful to us. Thank you.

[riseillyramos]

Omg i love you! Really wish my professor explained this well.

[sadiqaisha]

Thank you so much Arpan! I've never understood qPCR like I did in this 8 mins video!

[jaydebpal]

very good overview. can you please make a detailed one on melt curve analysis and primer designing

[sheratajayasinghe]

The explanation was really good. N easy to understand. Thank you very much most of my doubts was cleared ❤

[moleculartraveler]

This video is really well explained and helpful

[fathimasulaiman]

Best explanation about real time pcr.... Really good 😍😍😍😍😍

[djwuyef]

He is a legend ....ARPAN is a LEGEND ....

[Universe]

Love your channel and the way you make difficult concepts easy.
I had a question about primer property checking using Primer-BLAST.
I have a primer that contains degeneracy/wobbles and I want to check its properties such as Tm, self-complementarity, self 3’ complementary using the primer- blast…

However, when I use the primer Blast and search, it states that “ambiguity letters other than N are not allowed in the primer sequence”
So that forced me to replace all the degenerate/wobble letters with N.
Would that still make the property results correct? Am I still getting the right answers if they are replaced with N?
Thank you so much, I would really appreciate some insight on this, as I can not find an answer anywhere.

[ominibioentrance]

Good if the ct value of control and treated sample are same say 26 for both. Does it mean that the transcript level is same in both.

[spajder]

Very nice video, but it would be great if there was subtitles

[SaumyaSharma]

What is the need to do qpcr over pcr sir?

[harshithaanand]

thank you so much was very very helpful

[cutieaadi]

If I get a annealing temperature 66.4 and got single peak of melting curve, can I use that annealing temperature? While the Melting curve temperature range is 65_95’c ….what you suggest?

[sristi_]

Can qrt pcr be used to determine the exact number of viruses that have infected the sample?

[dimpabhalru]

sir abhi window period mai hu sir actually next month mai canada jaaa rha hu pr m glti se hepatitis C ke contact mai agya hu … sir confirm nhi horha….. pls btao sir kuch 25 febuary ko needle use ki thi hep c postive ldke ki sir fr 10 din baad hcv pcr rna quantitative test krwaya …. Usme not detected aya … sir plss btao next test kab karwau ki accurate aajaye jldi mne canada jana h..
pls help sir🙏🙏🙏