rtPCR animation

RT PCR animation - This lecture explains about the RT PCR also known as the real time PCR. Realtime PCR is a technique of amplifying DNA fragments with polymerase chain reaction and along with the PCR. it can help us to monitor the concentration of amplified DNA Replication in real time with the help of fluorescence emission. Real time PCR or RT PCR uses fluorescence resonance energy transfer or Fret to detect the fluorescence generated from the DNA amplification.
Animation source is - Sumanas Inc.
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Thank you for watching the tutorial on RT PCR animation.

[clowdier]

I think you've mixed up the nomenclature a bit. The video is about is qrtPCR (or qPCR) - quantitative real time polymerase chain reaction. RT-PCR stands for reverse transcription PCR, which is a different method from qrt-PCR, involving no fluorescent dyes as RNA is electrophoresed and Southern blotted :)

[souravpakrashi]

Heading is rt- PCR. rt means reverse transcriptase mediated PCR. But the matter is based on Real Time PCR or qPCR

[MDZ_]

I'm a med student and rt-PCR it's a pretty used acronym which stands also for Real-Time PCR or qPCR too. I think there is big scientific misunderstanding.

[marinahovakimyan]

Thank you so much
This was very helpful

[MrMetalHead]

so it seems like your quantitation is dependent on having excess amounts of probe. How do you account for nonspecific DNA binding due to having excess probe?

[hemabhardwaj]

I have read that flourescence is shown when the probe paired with the dna acc to u it shows after the degradation of probe?

[shahirkhan]

Sir,

In real-time PCR, Why Extension occurs just after annealing?
Why the primers/probe are added first not the dnTP's. What makes dna polymerase not working then?

Does Dna Polymerase always need s a primer(sequence) to start it's job?

[bipashabhattacharjee]

Hi! You rock but your title is still misleading 2 years from now. It's qRT, not RT PCR you're talking off!

[drparks]

Please change the title. This is how students get so damn confused.

[acemax]

Many thanks for yet another amazing educative content. Helped in obtaining a deeper understanding of the topic.
Could you please explain what "background level" means?
Thanks in advance!!

[kareshma]

Thanks alot, can you please exlplain more about how to read the PCR result from the curve and why sometimes results not clear and sample repeated

[katp]

Hello,

what dyes have this quencher and reporter probe together? I know SYBR green doesn't.. or does it?

[Floyd]

Does the probe only ever bind to one strand of the target DNA?

[esanau]

Why don't you change the name of your video? It's misleading

[shacheeswaraj]

This is not RT-PCR. You are teaching quantitative-PCR (qPCR).
Kindly change the name of the technique.