Real time PCR

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A quantitative polymerase chain reaction (qPCR), also called real-time polymerase chain reaction, is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR), which is used to amplify and simultaneously quantify a targeted DNA molecule. For one or more specific sequences in a DNA sample, quantitative PCR enables both detection and quantification. The quantity can be either an absolute number of copies or a relative amount when normalized to DNA input or additional normalizing genes.

The procedure follows the general principle of polymerase chain reaction; its key feature is that the amplified DNA is detected as the reaction progresses in "real time". This is a new approach compared to standard PCR, where the product of the reaction is detected at its end. Two common methods for the detection of products in quantitative PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence to quantify messenger RNA (mRNA) and non-coding RNA in cells or tissues.

qPCR is the abbreviation used for quantitative PCR (real-time PCR).[1] Real-time reverse-transcription PCR is often denoted as: qRT-PCR[2][3][4] The acronym "RT-PCR" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention. Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia.

[whydizz]

How do people not understand what you're saying with your accent? You're English is much better than some of the other Indian lecturers. Thanks for taking the time to upload this!

[lokitekq]

Finally found my molecular biology plug on YouTube.😁🔥🔥

[adekunlemaryam]

Thumbs up. The way you explain this things is

[hanaparos]

Very informative and CLEAR.  Thank you Mr. Bhattacharjee!

[deepthirao]

Excellent presentation! It helped me a lot.. I didn't have any problem understanding his accent.

[xHaniffax]

All your videos are very helpful! Thank you!

[pragyasharma]

thanks for being such a versatile teacher

[BilalAhmad-lfnp]

bro your lectures are really worth listening ..well done

[zdenkajp]

Thank you so much, the explanation was complete and well delivered .

[sivachaitanya]

wow...prachondo bhalo.. ei videota khub informative.. anek tekh kore bolchilen... ami M.S porchi IICB jadavpur the.. amadir QRT-PCR ache.. ami anek bar korlam ey technique.. kinthu basics chiney na.. ekhun jantham.. bcz of u..
  

[piko]

Thank you very much for sharing, it is very clear and informative. Congratulations for the good work!

[tommasopusceddu]

At 24:00 you describe Scorpion primers not molecular beacons, molecular beacons are like scorpion primers but without the blocker and the primer. Molecular beacon probe will bind a specific DNA sequence and doing that the reporter and the quencher get away producing fluorescence.

[phearmetoo]

that is great! finally, I understand qPCR

[samabayramiazar]

super clear and comprehensive! thanks a lot

[jesushernandez-gwqj]

Thanks again for the great video. Pretty easy to understand.

[misschaitali]

Thankyou sir for sharing your knowledge here. It helps us a lot.

[Tiyaraify]

Can someone tell me what the basic mechanism of the PCR technique is, please? Thanks in advance

[sakiyamir]

plz explain Serial analysis of gene expression

[dhrubanandamukherjee]

Sir western blot is for proteins and PCR is for DNA... how can we compare the sensitivities of these techniques??

[anaisoceane]

hello!please how can i know wich technique do i have to use, our teacher gave us an abstract and she asked about which technique do we have to use in that and that experience,