How to count cells with the Neubauer Counting Chamber

Nowadays most labs use automated cell counting systems to determine the number of cells in solution, although the classical counting with the Neubauer counting chamber is still in use and it is rather easy to work with it.

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NoMBe - Interplanetary Alignment (YouTube Audio Library)

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Anna R.

#Counting #cell #Neubauer

[sdqssku]

wow, it was so easy to understand by this video.

[fflyrol]

Good day, pls i dont understand the calculation aspect of the video, pls can u explain in details of how u did the calculations. Thank you

[Ali-sxcn]

Wait i saw a different video saying u can't count the cells on the lower and right corner lines but u are saying u should im confused now but that guy was using some trypen blue method which he called it

[etsxhbk]

Why you have two Factors at the beganing of the vieo 10 and then 10 exp 4? Please explain me.

[sayamk]

Hi! Thank you for the video! I have a question regarding the cell counting, cause in some labs they only count 4 corner squares, but here it says 5. Does it depend on a cell type? For example, should I use your method in counting HEK 293 cells (Human embryonic cells)?. Thank you in advance!

[EmekaIloegbu]

Thank you for the presentation however your explanation at the 2 min and 27 mark about which cells to count and what shouldnt count isnt entirely accurate. I had to listen to it a few times but counting the cells in the blue boundary (bottom and right) is inaccurate because you dont know if it truly falls within that square. However if you are counting cells at the top and left, there are no other squares on the outside of those boundaries so it is safe to include those cells in the estimation. I am open to understanding this more and look forward to your response. Thanks

[shrikantkirwale]

if the count is 50 (avf of four) then multiply by 10 is 500 so we have 500 cells per uL

[commoninternetperson]

When counting the cells do you distinguish between viable and non-viable cells?

[coby]

What is the reason for not counting the white squares?

[PixelstarWASD]

Who needs a fertility lab when you can just DIY?

[hohpymv]

Please I don't understand how 10 to the power 4 came about

[mohamedjedoui]

The counting is not right if you count diluted cells. you have cells per ml and the total number of cells is: cells making a total number of 1 million cells in 10 ml