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Counting Cells
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In this video we will show you how to count cells using a hemocytometer. This protocol begins after our cells have been trypsinized and collected. To learn more about this and tissue culture in general, please see our video on Getting Started with Tissue Culture.
We’ve broken this video up into two major components for ease of use; the laboratory work involving setting up the hemocytometer and counting the cells, followed by the calculations you’ll need to determine your cell density.
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0:00 Intro
0:41 Materials
0:58 Laboratory Protocol
1:41 Counting Cells
3:18 Calculating Viable Cells
3:56 Cleanup
We’ve broken this video up into two major components for ease of use; the laboratory work involving setting up the hemocytometer and counting the cells, followed by the calculations you’ll need to determine your cell density.
______________________________________________________________________________________________
0:00 Intro
0:41 Materials
0:58 Laboratory Protocol
1:41 Counting Cells
3:18 Calculating Viable Cells
3:56 Cleanup
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