Counting Cells with a Hemocytometer

There can be tens of thousands of cells in one milliliter of culture medium. So how are cells counted?

The process requires diluting the cell culture, dying the cells, and loading the cells into a hemocytometer. A hemocytometer is a modified microscope slide with two chambers. Each chamber is divided into 9 squares. There are specific guidelines for counting cells within the squares.

In this video, we'll demonstrate the process of counting cells using a hemocytometer. We'll also work through some of the typical calculations that accompany cell counting.

Our hope is that after viewing this video, you will feel more confident and be better prepared to try counting cells in a hands-on lab experience.

This video was filmed on location at Rowan-Cabarrus Community College.

[MrAllacazam]

i wish every single labs out there had beautifully edited constructed with great explanations like this... would definitely make uni life way more interesting... amazing video, hats off!!!

[shingonakai]

Your videos have given me great teaching aids to students who are new to cell culture! Thank you!

[mdumaguing]

I've watched so many videos on how to count the cells and you are the best ever who explained it properly, I can understand you very well with your speech and you explained it properly, thank you,

[hellofriends]

Very good video, but one mistake. At the end, she says that 94.7% of the 216, 000 cells/mL are viable. However, she uses the number of viable cells (54) to calculate average number of cells/square. If she had used the number of total cells (nonviable + viable, or 57) to calculate cells/square, then in the end it would make sense to take the final number and say that 94.7% of those cells are viable.

[Obalatan]

Excellent work. It's crystal clear and systematically explained. Thank you for putting this video up.

[amailyakter]

excellent!! today I got the total process of how to calculate cells, thank you Miss for your wonderful video

[MarianaDiaz-vowb]

WOOOOW, It's super clear!, Thank you very much for your support!

[BrandonRosenstrauch]

Wow, this was well put together. Great job!

[kerensahardesty]

What crystal-clear instruction. Amazing!

[palsnicky]

Really easy to understand and great use of simple graphics, I'm just looking into yeast counts for brewing. If I can follow this anyone can, thank you.

[lilicsb]

This is the best video about the topi... so easy to understand :) Thank you so much!

[ahmedsalah-ocvl]

really amazing
excellent photographing ... direction.... sound effect... commentary ... subtitle .. perfect timing.
science is different actually

[masterpee]

Thank you for saving me. No video could explain it better.

[mikestout]

Thank you. This is just what I needed.

[EDUARDO]

Thank you very much, now am ready for lab class tomorrow here at MD anderson

[ramlahassan]

This helped so much! I can thank you enough! Crystal clear! Keep up the great work:D

[ahmadtalsaadi]

Thank you, this is the best presentation in this regards

[Iinachanelk]

Really enjoyed it! Excellent quality throughout! Thank you so much! xoxo

[aishwaryaray]

Thanks for such a great explanation ...really worthy enough

[jimines]

Wonderfully articulated and easy to understand, better than many of my teachers.