How we test for SARS-CoV-2 - RT-PCR (Reverse Transcription PCR)

Hey scientists,

today I will present you a technique which is used for the testing of patients whether they are infected with a certain virus or not. This is for example used in the Covid-19 Corona virus crisis right now and the RNA extracted from patients undergoes exactly this mechanism described here.

This literature here was helpful for me:

Video about PCR (Polymerase Chain Reaction):

See you next time!

Cheers,
Henrik

[henrikslab]

In the Covid-19 Test:
The primers are designed so that they can just bind to RNA with the exact same sequence of the virus. Then DNA is amplified. Otherwise if the primers don´t match with the RNA, no amplification takes place...

[haidarrafid]

I don't know how to thank you... this is what I've been looking for, god bless you! danke schon!

[yogeshlakshman]

Explaining the concept in simple terms is challenging. And you did it great sir🎉
Thank you

[alessiagiarolo]

And microbiology homework is done with no stress <3

[Uradur]

The SARS-CoV-19 RNA is a genome of this virus and it is also mRNA, on wtich six virus protrins are synthesized

[FilterYT]

This was very well done, thanks for the clear explanation. I think it would be nice to explain more (I'm going to look through your library to see if you have already in another video) how the identification stage is performed. I think I understand but don't feel like I could use this video to help people in my life with the questions I am getting. Thanks again!

[trappsterbeats]

Your mRNA sequence is wrong. mRNA is synthesized from 5' to 3'-End the mRNA sequence shown at 1:41 is reversed which is wrong.

[willemkaret]

The company I work at is in the vital sector. When testing availability increases over the coming weeks, we will have to decide if we will test staff for SARS-CoV2 and antibodies. Thank you for this refresher.

[geonerd]

I wish you'd take the time to explain the HOW more than the WHAT.

[wewrestlenot]

I thought the creator of this test said it was not suitable to test for infections diseases???

[dobby]

absolutely amazing video, so easy to understand!

[MohamedAli-bnin]

Why are the individual bases of the cDNA not complementary to the mRNA but rather just a DNA sequence? Like, the cDNA has only difference in bases such as U with T and T with A's. Other than that, on the cDNA, it's a C for a C on the mRNA and G for a G on the cDNA? I thought it would be complementary and a C on the mRNA becomes a G on the cDNA for example? Apparently from what i checked this is not the case? Can you explain please

[nobutaka]

Why do you make multiple copies of the same segment? Don't you want to just make one or two and move on to the next segment so you get it all reverse transcripted?

[devikagiri]

This is Real time PCR my friend, completely different from Reverse Transcriptase PCR

[Hollytitan]

are the primers for RNA and DNA the same ?

[nels]

Great video! One point of clarification here is that SARS-CoV-2 is the name of the virus, while COVID-19 is the name of the disease. So it is really a test for SARS-CoV-2 because you could test positive but be asymptomatic.

[NivedGopi]

Why do we make cDNA since the 1st step of PCR is denaturation which make ss DNA?

[debbiejoyable]

Hi, what software did you use for the illustrations?

[ISTARI]

Can someone help me. I'm not that good with science but I roughly understand. During the denaturation of the cda, you use taq polymerase to elongate the the rna strand or the complimentary strand which binds to the rna strand? Also, why can't we detect covid by just amplifying the cdna?

[ysxydx]

Where does the primer come from ? I n the first step annealing
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