Maxam–Gilbert DNA sequencing - Biology tutorial

Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1977–1980. This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides.

In this tutorial/lecture we will look at the Maxam-Gilbert sequencing method. We cover some topics important for classes such as Chemistry (High School and College Levels), Biology, etc.

If you have any questions, don't be shy!! We hope we are able to clarify this topic.

Enjoy!

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[canbeanyoneforfbi]

I'm russian, but I understood your lesson better than the explanations of all russian-speaking bloggers, so thank you <3

[lenonre]

clearest expanation of Maxam Gilbert method!

[JENNIFERLORENALOPEZHOYOS]

I cannot believe i never saw you before, THANKS

[EshaanBanerjee]

Very good explanation Sir, but in the last place where you have show the gel electrophoresis data, there is a mistake.

According to your DNA sequence, the shortest sequence should be in the G+A line of the PAGE.

But, you have put it on the C+T line.

Leaving this, its the best video on youtube for this topic.

[zizojaezekeom]

❤ Thank you i understand everything just a question, the DNA we sequence is simple strand right?

[emyyahia]

Hi..thank you so much u helped alot ❤
But i have a question pls ..how do u know the very first nucleotide?! It wasnt in the gel

[Hugh_Mungus]

Do the 4 chemicals cut on the 3' end or the 5' end or both or does it depend on the chemical? It doesn't seem to matter since the P isotope is always in 5' but I want to know

[borrapandu]

wow, very nice and simple to understand. THANK YOU

[Music-zxpo]

Hi, thanks for your video! I have a question though, why wouldn’t you just use A instead of A+G?

Also, how is it possible to use one strand of DNA for multiple cleavages, since it literally requires each sequence end to be destroyed?

[omarmohamed-pdpw]

I have a question if we apply the chemical that cleaves at G wont it cleave at both G sites at the same time so a GCACCATCG be formed disrupting the electrophoresis

[Captain__T]

Wow very helpful. You posted this on my birthday🥰.