What is Sanger sequencing?
A conventional method used since 1977, is still used today
In this we denature the DNA, by increasing the temperature. Cool down to cause primers to anneal. DNA polymerase then extend the DNA fragments until a fluorescently labeled dideoxyribonucleotide is reached. The chain is terminated.
The colour of the florescent indicates the base at which the chain is terminated.
When the DNA fragments are separated by size, we can determine the sequence of terminal nucleotides and determine the sequence of DNA/genome
hifza
Spectacular explanation! It’s amazing how you simplified it thank you
leenahmed
Very concise yet gets the concept across in an excellent manner which is very easy to understand! Thank you!
AliShaikh_
Wow. Amazing explanation, it really helped me understand how genius this method is.
beckseamons
Thanks a lot! This was easy to understand
wahhajmustafa
Great video! One thing--I think it's misleading to say the ddNTP is "randomly" inserted. It's inserted according to Watson-Crick rules.
nfinfsu
Wait.. isn't this the procedure for Polymerase Chain reaction?
erzascarlet
I don't understand how we get to know about the sequence and why we are terminating it with ddNTPs??
Can someone help me with this?
rabia
@0:48 ... is missing a hydroxyl group which allows the next nucleotide to bind - you mean it does not allow the next nucleotide to bind, thus ending in termination...
