Maxam gilbert DNA sequencing method

This DNA sequencing lecture explains about the Maxam gilbert method of DNA sequencing or chemical DNA sequencing.
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[praveenvemuri]

Its a good try, but there are few things which has to be dealt for better understanding like:
1. what chemicals are used specifically to cleave a certain nucleotide
(G-DMS, A-Formic acid, C-Hydrazine+NaCl, T-Hydrazine) together with piperdine to cleave the phosphodiester bond of modified nucleotide
2. How to make single stranded DNA fragments
(heat denaturation or using helicase)
3. Dimer formation are in combinations of A+G and C+T
If a band in the DNA sequence appears in both the G-reaction and the G+A-reaction lanes, then that the nucleotide is a G. If a band in the DNA sequence appears only in the G+A-reaction lane, then it is an A. The same decision process works for the C-reaction and the C+T-reaction lanes.
4. What quantity of sample DNA is required for sequencing
(if not sufficient, have to run PCR or do cloning to amplify)
5. What about the left part of DNA strand after cleavage
(they cant be visualized, due to lack of isotope)
6. What percentage of gel is preferred
(6% polyacrylamide gels are used, bcz they are too sensitive in differentiating the DNA bands with 1bp size)

[mpv]

I just realised that I've been passing my entire uni thanks to you but never said a proper thank you so: Thank you! (=

[postmorton]

I'm doing an essay on genomic analysis, and these videos on sequencing are amazing for a proper, scientific understanding of how sequencing has developed over the years. Thank you so much for the clear and helpful video!

[sohailvet]

I am a veterinary student one veterinary university in we dont have any teacher like u u very much❤️❣️

[ananyayadav]

I came across this video, and may i say you are the best youtuber teacher we have got
Hands down sir, you are brilliant

[ejazkhan]

no need for short videos....you are doing great job... because it is helpul for begginers, , , if you start to make short videos ...then the beginners will not understand....thanks alot...very

[romi]

seriously... The Best Lecturer Out there.. I find it so effective.

[drsahivlogs]

My best BIOLOGY teacher over whole youtube

[RosemaryUkamaka-ieuc]

This is so helpful but I almost got confused at the initial result interpretation but thank God, I later understood it.
Thank you Shomu Biology

[alwinaanam]

indeed its topic bt after watching twice /thrice i can understand for sure..

[AzhunkWorld]

Interesting and very clear explanation. don't bother about the time because it's very interesting and understandable. Thanks shomu!

[fatimariaz]

when you write down on board that method is good for understanding and due to only this difference between you and other you tubers i was watching your videos

[tamannaparvin]

Thanks for this video. It's really helpful for topic is in my syllabus....thanks a lot

[CringeDealer]

Really really helpful... Understood the topic thoroughly... ☺️

[KountayDwivedi]

Awesomely explained. This is my first video on Maxam-Gilbert, n you cleared the concept seamlessly. I won't go into technical details, I will just say that concept-wise this lecture is awesome.
:-}

[bamd]

but what about the first nucleotide ? how to know it?

[nohabassiouny]

you have a simple way in teaching and presenting the idea

[aakaankshavangala]

1. Not Chemical Synthesis. It is Chemical Cleavage (two completely opposite meanings)


2. First nucleotide determination without which DNA sequencing is incomplete. From my understanding, you are supposed to initially cut/cleave the DNA sample with a restriction enzyme. From this you get to know where the DNA is getting cleaved (as you know the restriction sequence of the enzyme). This is then followed by the steps explained in the video.

[nemapusai]

Very nice sir ! no words to tell and your classes helped a lot. thank you soo much sir

[princecudjoe]

that was some top explanation man. thamks!