DNA Sequencing - 3D

This 3D animation shows the basic steps in the method of DNA sequencing that was used during the Human Genome Project.

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Animated by Polymime Animation Company Ltd.

[ShukraanLife]

For those who didn't understand: Even though the terminator bases are added randomly, the process is repeated enough times that there are multiple copies of each length strands and all possible length of strands are produced enough times. As a result, when the electrophoresis is finally done the strands get arranged in short to long order, the last/terminator base of each strand marking the end of each strand. Thus when you put together the end base of each strand, you get the original sequence of that original piece of dna. (Remember the beginning portion of each length strand here is always the same because the primer binds to a specific location only, so the produced strands will look like this hypothetically: ATT< ATTC< ATTCG< ATTCGT etc.)

[goldenmeerkats]

Whoever came through and made this vid is a g straight up

[donnam]

Do you know how long I've been looking for this explanation? 3 hours!

[sev.salahshour]

It was so amazing and interesting. Every time I hesitate about choosing med/bio for my future studies I watch these kinds of videos and get reminded what an amazingly beautiful world this field is.

[THE______TRUTH]

Damn, that's the best explanation I've seen of this.

[haroonrashid]

Awesome presentation👍.
These terminator bases are dideoxynucleotides, which lack both hydroxyl groups at 3' and 2' carbons of sugar, and hence can't make phophodiester bond i.e terminate dna synthesis.

[vulcanodong]

This is my first time to know how to DNA Sequencing. A really smart idea

[matsreinderink]

Biology would be a lot easier if we didn't have DNA.

[asmaasif]

أنا مهتم جدًا بعلم الوراثة ولكني أجد صعوبة في دراسة الحمض النووي. لقد جعلت مقاطع الفيديو الخاصة بك الأمر سهلاً للغاية بالنسبة لي. شكرًا. يرجى الاستمرار في إنشاء المحتوى الخاص بك 💫

[phlbyss]

Thanks a lot...I never understood DNA sequencing in my study life...now, Alhamdulillah, I got it...

[isabellamichaelis]

PLEASE MAKE MORE VIDEOS THIS JUST SAVED ME ON MY EXAM

[NixSiow]

Very nice explanation. Very smart techniques and learnt a lot about DNA sequencing process. Thanks! Looking forward for more of your video.

[DanyalShabirr]

THIS IS WHAT SMART STUDY ACTUALLY LOOKS LIKE.
THANKS BUDDY😊
WHEREVER YOU ARE I HOPE YOU WILL BE ALRIGHT.
AGAIN, THANKS FOR THIS WONDERFUL MASTERPIECE.
THIS IS WHAT YOUTUBE WAS ACTUALLY MEANT FOR.

[manuelsokolov]

So, If I understand correctly.
1. Cut DNA by pieces and add each piece to a sample of DNA (plasmid)
2. Pass each piece that was added with plasmid DNA to a bacteria that would multiply. (How do we not get repeated copies that we don't need where?)
3. Add free DNA bases (A, C, T, G), DNA Polimerase, DNA primer and modified DNA bases that will act as terminator basis
4. Then we raise the temperature to 90 for the spiral to spilt in two (like PCR) and then low again we split so that DNA primer can connect and the free DNA basis connect, until it finds a stop sequence in which it adds the modified terminator basis. (How are we keeping the sequence here? Aren't we combining two things that we created, the DNA primer and the plasmid DNA?)
5. Then we raise the temperature again so that we can split the sequence that we just found. And then repeat process 4, 5 for many times.(Again... Aren't we getting repeated sequences?)
6. Apply electroforesis in order to split these sequences by size.
7. Then we get all the sequences and use a laser to light up the terminating base. And save all the basis that light up, this way we get the sequence. ( Why do we only save the terminating base and not the sequence? And how are we sequencing a person DNA if we are keeping one terminating for each gene sequence found?)
Added some questions for each phase in brackets. Hope that might be also other people questions.
Best Regards,

[foal]

this is god level work. Its mind boggling how scientists have come thus far and developed the tools to discover AND manipulate DNA.

[islang]

PLEASE MAKE MORE OF THESE THEY ARE REALLY HELPFUL.

[achoice]

can we just have a moment of silence as an appreatuation for the Human Genome project and all who worked on it !

[vrashabhsugandhi]

Very proper 3D presentation an Very nice explanation of Sequencing DNA looking for more Nice videos of Genitics..

[alfishaakram]

It helped me a lot 😍this type of animation take a very short time, and preventing us from learning....so many books...in less time I understand more things😊

[renshe]

This video is actually a fucking godsend thank you yourgenome for making this video, and I will give thanks for future bio students, God speed.