Sanger sequencing

This is an animated video on DNA sequencing by Sanger's method.

Sanger sequencing method is based the use of dideoxy nucleotides in the DNA polymerizing reaction. The hydroxyl group attached at the 3' end plays a very important role in DNA polymerization reaction

during DNA polymerization, There is a reaction between 3'OH group and the phosphate of the new dNTP to be added.

in 1977, Frederick Sanger had a brilliant idea. if the 3'OH group of the nucleotide is absent, then there wont be addition of new nucleotides and the reaction would stop. The incorporation of dideoxy nucleotide and termination of polymerization reaction is a random process. hence the overall mixture will have several fragements that have experienced the chain termination. If the length of each fragment is known then the location of nucleotides in the DNA can be estimated.

[ericeckenroth]

Classic example of some random youtube dude better explaining a concept than my professor can

[Dann]

Just because you have a PhD in something, DOES NOT mean you are a good teacher. Thank you so much for this my friend.

[TakeitSleeezy]

Its crazy how things like this when taught correctly are so easy to comprehend. Professors/Teachers need to take notes from this guy!

[ginsnow]

this is the best video on Sanger's Method I've seen. I finally understand it now. Thank you so much!

[varshakashyap]

Best video on YouTube available for sanger sequencing.

[Atj]

I mean my professor made this topic so hard that I thought I don't have the brain cells to understand this. Tysm buddy

[saboo_tage]

My goodness, people really weren't joking when they said you're an amazing teacher. Aside from having my mind blown from how genius this technique is, my mind is also blown from how amazing of a teacher you are. Thank you ever so much!!!

[ghitabencheikh]

i just want to thank you, you explained this better than most of my professors during my pharmacy school years. thank you!

[djilnmk]

Make sure you realize that the sequence provided in the answer is the complementary sequence and not the parent sequence.

[hillarymorin]

I have been struggling to understand this concept, and this video was perfect!!! Thank you very much.

[LittleMissLonely]

This is hands down the BEST Sanger's Method video I've seen. So clear. Excellent work! Thank you

[dannaeparry]

The most clear and thorough explanation of Sanger Sequencing. I couldn’t wrap my head around how the sequence of the strands are determined but it is thoroughly explained in this video

[momo____]

My Microbiology exams are approaching and this video is a pure Bliss to me.This video is perfectly making my bases strong along with making me understand the whole topic which my professor failed to during a whole 1 hr lecture in my native language.

[crachhit]

Saved my life, i hate not understanding something but thankfully i found this video. Great explanation!

[agussschh]

this video made it so unbelievably easy to understand, geez

[wyytoxic]

this 6 minute tutorial saved me a full hour of time of watching my lectures, thank you.

[christianluensmann]

Vey surprised that I completely understood this on my first watch through. Such a great video, thank you so much.

[sorayachquiry]

so we're all here bc our professors can't teach anything during lecture :')

[ThePsychokazik]

that was life changing! I hope I will use all that information on my test tommorow. I want to say hi to my dear friend Dawid <3

[khush]

U just killed it ...rather than watching so lengthy and complex videos ..its far better and easy way to learn
:-)