3) Polymerase Chain Reaction (PCR) - Quantitative PCR (qPCR)

What is Quantitative PCR (qPCR)?
➜ Real-Time PCR or quantitative PCR (qPCR) is a PCR-based technique that is able to simultaneously amplify and detect changes in the amplicon concentration. Real-time PCR collects data during PCR amplification by utilizing fluorescence signals emitted by either special probes or DNA binding dyes.

For more information on qPCR and for a list of the sources used, please visit:

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[jyprevet]

I'm a korean veterinary student, and this video makes me deeply and clearly understand about qPCR

THANK YOU!!!

[nairacaraffa]

Direct, clear and specific explanation of fundamentals. Thank you...

[borgshadow]

nice video, good to study and remember what you have already learned (but not used for some time)

[shivakumarbelur]

Indeed, a wonderful explanation for all the audience to understand the complete process of RT-PCR👏👌👍
Thank you ABM🙏

[DivyaePrasad]

this makes the concept so easy to understand, thanks a ton :)
but at 2:30, inverse correlation doesn't mean that its equal to reciprocal of nucleic acid in sample! just a minor correction!

[gogomyu]

This video is really helpful!! Is there any video comparing those DNA polymerase kinds: Tfl, Pwo, Tth and Pfu with Taq polymerase?

[bt]

In the video, it is mentioned that a drawback with the intercalating dye method is the binding of non-specific sequences. But it also mentioned that the melting curve analysis enables the user to distinguish the amplified products through plotting fluorescence as a function of temperature. So does the mean we can use the melting curve analysis to differentiate between the binding of specific and non-specific sequences as they should have different Tm?

[DrAbdelmoneem]

Thanks for this awesome video, probably my second comment on youtube :D and the first was yours :D

[Notarealusersname]

Are the labeled 5' to 3' strands flipped around the 5 minute mark?

[MultiSciGeek]

I have zero understanding on any of this, and after searching for an hour I finally found a video that helped me out!
A question though: what is a DNA dye and how does it get attached to your sample? So from what I understand a viral DNA sample is taken and PCR is done on it, and now I know that at the end this photodetection is done. But what is the step between the two? How does the viral DNA sample emit light for a machine to detect? Second, I saw in an article that said _a patient had a PCR CT value <34 and was discharged._ What exactly does that mean? I'm guessing: very little viral DNA indicating a less severe infection, the patient's condition has improved. Is that correct?

[victoiretcheuleu]

Please can you tell me the price of this machine?

[kristingallo]

Hello can you tell me how accurate fetal gender screening is with this method after 20 weeks if you get a female result?

[shaheenh.]

If only the background music wasn’t there 😭😭😭😭