Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR) is a widely used method to make many copies of a specific nucleic acid segment in vitro.

The amplified DNA segment can be used in wide range of processes such as in the Human Genome Project, in DNA fingerprinting, in the detection of viral particles, in diagnosis of genetic disorders and so on.

PCR requires DNA template, Taq DNA Polymerase, DNA primers and free nucleotides.

PCR carried out in 3 steps and in repeated cycles. These steps are denaturation, annealing and extension. Usually 25-30 cycles are required to produce a sufficient amount of DNA copies.
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