Polymerase chain reaction (PCR)

Polymerase chain reaction (PCR) is a technique used in molecular biology for DNA amplification of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the thermophilic bacterium Thermus aquaticus. This DNA polymerase enzymatically assembles a new DNA strand from free nucleotides, the building blocks of DNA, by using single-stranded DNA as a template and DNA oligonucleotides (the primers mentioned above) to initiate DNA synthesis.

[cristalserrano]

I'm from mexico and I love your videos because I understand much better. Your're the best, thank you so much.

[MatthewChoDotCom]

appreciate the video. but that bg music triggers

[fatmaftima]

Thank you so much I'm from Algeria you made it so easy

[zcetin]

No music pls, still good explanation, , :)

[aaquib]

Why doesn’t the template also anneal back when the temperature is turned to 55-62C (annealing temperature of primers)?

[nooribrahim]

Thank you, you made it very easy Thank you much 🤝🏻🤝🏻🤝🏻💗

[ekhushimanzoor]

Really yr u r genius inay tym mai smjaya thnkx dea thnkx a lot

[Pionike]

How does the DNA polymerase knows when to stop the elongation process?

[YTspeurder]

Maybe a stupid question :) but why do these primers chose to bind at the correct spot?

[prakashdensil]

Love from Chattisgarh india 👍🙏😘🇮🇳😘🇮🇳😘🇮🇳

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