PCR | Polymerase Chain Reaction | Video 3

In 1986, a technique, called the polymerase chain reaction (PCR), was developed. PCR is a rapid method of DNA cloning that extends the power of recombinant DNA and in many cases eliminates the need to use host cells for cloning. PCR is a method of choice for many applications, whether in molecular biology, human genetics, evolution, development, conservation, or forensics. PCR Reagents are pre-requisite of reaction. Reagents include Template DNA, Primers, DNTP's, Taq Polymerase, PCR Buffer and MgCl2 which is a cofactor for Taq polymerase.
PCR is performed in three steps.
1) Denaturation
2) Annealing
3) Extension or Elongation.

[samreenansari]

Sir your teaching skill is amazing, during exam i forgot everything about PCR whatever i read from book only remember your lecture.

[MaryamAmin-ziuj]

Thanku bhai😊in Ramzan when you sit in study and watch this video..all thing clear❤

[humaahmedkhan]

Sir ap to highly qualified hen n teaching skull b best hen 1st time hee samj aa jata hey....
Sir ne class ma samjhaya tha but bhol gai thi....
Magar ap ne Jo samjhaya wo bohat details ma tha n easy he.
ALLAH taraqqee de apko ameen

[AMINABIBI-jh]

You are my fvrt lecturer, keep doing your work as it 😊😊☺️🌺

[shariq_mewati_]

Walekum assalam sir perfect explanation thanku so much sir

[pavantripathi]

Loading of the primer is not annealing, rather the process of making primer from the complementary strand is annealing. Which takes place at 54°C.

[MkhanSep]

I am watching this vedio before half hour in starting my paper😅

[Extraordinary]

My eyes seeing indian tricolour in the strand of dna🧡🤍💚🥰

[ahsanaliabbasi]

Ye Long question h class yad kr lo ye video kam ki h 😀

[prafull_gzp]

teacher ho aap.... thoda shirt pahan liya kriye... yha pe fashion show aur bodybuilding competition nhi chl rha.. waise padhate aap acha hain