Polymerase chain reaction (PCR) | Biomolecules | MCAT | Khan Academy

Introduction to PCR (polymerase chain reaction).

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[perpetualplantstudent]

We need a man like this in every molecular biology class, questioning LITERALLY everything. Because EVERYTHING needs a little further explanation Ms. Sawa!! Lol

[nokwandandlovu]

I LOVE THIS WAY OF EXPLAINING SOMEONE WHO KNOWS AND ONE WHO DOESN'T KNOW THAT WELL

[jessemaretzki]

Hi Sal! I am studying pursuing my undergraduate studying molecular biology with a presentation on Real time PCR tomorrow. I really think this is an amazing process especially with respect to the forensic applications! Thanks for this video, it kind of summed up everything I have already covered so I feel really ready! Khan academy has been great since 8th grade and I am still using it now studying for my MCAT so thank you from the bottom of my heart!

[TsangChiYim]

I am a layman but I can still understand PCR completely through this awesome video. Thank you Khan Academy!!!

[zenaguerrero]

Always love how you guys break down the extent of knowledge to be understood :D

[milo]

Omg this is guy is awesome how clear he is in his explanation thank you thank you thank you comme to teach to our community college in Glendale Cali please!!!!

[nathanielscreativecollecti]

Best explanation I have seen so far! Yeah Khan Academy!

[ariatnaserrano]

Literally all the questions I had you answered wow thanks.

[paolamacario]

love this.. have a test this friday and it was hard to understand my textbook

[darisberdavis]

Every video that I see with this man’s voice…. I automatically know that I will learn something ❤. Love it

[kmaster]

I don’t know if the video mentions it but you can also use pcr to generate multiple copies of a mutated strand by introducing a primer containing a mutant base then favoring only mutant by introducing endonuclease to remove parent strand (mostly like nucleases selecting methylated strands) then repeatly coloniezes bacteria until you got a mutant

[TheLizardWizard]

Wow thank you for the lightbulb moment

[marchenkogirl]

I had such a hard time understanding this material until now... thank you!

[makemelotsotetsi]

fascinating..I am studying Botany at the university of the free state, this video was really helpfull

[shakilarasa]

Thank you I have a question What is the purpose of the positive and negative control in the PCR reaction?

[khayalamimahlalela]

Glad and grateful to witness your passion in sharing your knowledge. Bless up!!

[ejikechristianogadinma]

I had a great time listening and watching 👍

[valorijoy]

To be clear, you do NOT exclusively have to use Taq polymerase. My lab uses Phusion. What matters is that it is thermostable.

[Wfb_DVM]

How do you know what exact region you want to make copies of from the original template?

[djsmith]

Can someone explain to why it must be cooled afterwards separating the DNA? And then reheated for that last step?