polymerase chain reaction ( PCR) | What are the 3 main steps in a PCR reaction?

This video not only describes the basic principle behing pcr but also the story behind its discovery.
this video would describe What are the 3 main steps in a PCR reaction? What are the major components of a PCR reaction?; What are the chemicals used in PCR? ; What are the 5 main ingredients in a PCR?, What is the product of PCR reaction?.

[AmitThakur-pyte]

Hello brother i am your very old follower.
How to identify resistance gene using pcr after antibiotics resistance profile.
Gene sequence is like available at ncbi we can design primer from primer blast.

[homaehsan]

hello, I have a question regarding PCR and non-specific amplification. we see a band in our negative control ( which is water), Do you think we can explain it as a non-specific amplification? we have changed everything and the suspicion for having contamination is low. what do you think?

[pratitidas]

How can i avoid the smearing problem? What is optimum template concentration should use?

[jabifatma]

Can u explain how Ori of bacteria can be identified using PCR???

[ДмитрийГолубев-юу]

subtitles are automatically generated in Japanese, can you replace them with English?