Mechanism of Recombination

TRANSCRIPT: A common technique in genetic engineering is to insert a new gene into a loop of bacterial DNA called a plasmid. The molecular tool used to cut DNA is a restriction enzyme such as EcoRI. The enzyme has a precise shape that allows it to run along the groove of the double helix, scanning for the base letter sequence G A A T T C EcoRI then cuts the plasmid at this specific point... ...allowing a new piece of DNA to be inserted. When it cuts, EcoRI leaves a sticky end, which helps the new gene to attach. The joins are THEN stitched together by another enzyme called DNA ligase. The genetically engineered bacteria is then grown in a culture medium. Very quickly, large numbers of the bacteria can be produced, each with a copy of the inserted gene. The bacteria duly manufacture whatever protein the gene codes for, and so the desired product is produced.

[DNALearningCenter]

Visit us in Cold Spring Harbor, Brooklyn, or Sleepy Hollow!

[alexspike]

This video made me genuinely uncomfortable, 10/10 explanation

[DaringGamerYT]

Short, yet informative. Thank you for this short animation

[DangerOfLaugh]

Important to mention that the restriction site (the sequence where the enzyme cuts) has to be palindromic

[charleyjr.iriarte]

Thank you for the teaching of mechanism of recombination.

Have a blessing.

[DNALearningCenter]

You're very welcome, and we're glad it was helpful to you! :)

[SarahKate]

SOOOO neat! I'm also learning about this... the animations accompany the textbook readings, really nicely.

[hmmmmm]

i'm learning this stuff for biotech right now, and this video helps a lot. thanks so much! ^_^

[shadow_of_thoth]

The miracle of life always fascinates me. How wonderful are these chemicals which come together in such a way... Just amazing.

[thetoonarmy]

This explains it perfectly, especially for GCSE biology level !

[ozzie_goat]

You spin me right round baby, right round, like a record baby, right round, right round.

(Couldn't pass up that opportunity.)

[varung]

@DNALearningCentre I wonder why do we need ti plasmid if it can be ligated regularly

[djg]

cut, stitch? what does that mean? i wanna know about bonds, molecules and the chemistry of it all!

[morrowind]

How does the ends(sticky/blunt) affect the use of ligase? ;O

[roreso]

^^ awesome ... but need mooore details !!!

[cedrix]

Is it easy to do? Or does it require advance machinery or years of trials and errors?

[zedooncadhz]

How do you know where to cut the DNA from which you are extracting your desired gene? Wouldnt you have to know the genome and specific areas that are operons? Do you have to remove whole operon or just the gene? How do you find a restriction endonuclease that will cut at the perfect positions so that you isolate the gene?

[Mudang_pullo]

Very nice animation..👍 but try to explain in more details

[nurse.lauren]

Does this video relate to Genetically Engineered Microoganisms?

[alexapricewhelan]

This video does not show recombination. It shows ligation. Also, the singular of "bacteria" is "bacterium".