CONJUGATION, TRANSFORMATION, TRANSDUCTION (HORIZONTAL GENE TRANSFER)

Bacteria engage in horizontal, or lateral, gene transfer, meaning that genes are exchanged between cells of the same generation. In contrast, vertical gene transfer occurs when parents pass genes to their progeny. The three methods of horizontal gene transfer that bacteria employ are conjugation, transformation, and transduction, with conjugation being the most common. Transformation and transduction typically take place between bacteria of the same or closely-related species. Conjugation is encoded by plasmids or transposons. Plasmids are circular DNA sections that replicate independently of chromosomes. Transposons, also known as transposable elements, or jumping genes, are mobile sections of DNA that can move within or between genomes. Conjugation with plasmids is more common than conjugation with transposons. Conjugation requires direct cell-cell contact. A conjugative plasmid or conjugative transposon is self-transmissible – in other words, it has all the genes need to connect with another bacterium and transmit itself to another bacterium via conjugation. We still don’t know how the majority of gram-positive bacteria achieve the intimate association of cell surfaces required, however, in gram-negative bacteria, this typically involves a conjugation pilus, also known as an F pilus, or a sex pilus. The conjugation pilus binds the other bacterium, then retracts to pull the two cells together. Once a bridge is formed with an opening between the two bacteria, they are now called a “mating pair”. A nuclease breaks one strand of the plasmid DNA at the oriT, or origin of transfer site. The nicked strand enters the other bacterium while the other strand remains behind in the donor. They can now both produce a complementary copy of the plasmid so the DNA is double stranded again. Now each bacterium has a copy of the plasmid and both can make a conjugation pilus! Note that in a population of bacteria in which some have a conjugative plasmid and others do not, eventually all cells will acquire a conjugative plasmid. The enzyme transposase catalyzes cutting and resealing of DNA during transposition. Note that once mating pairs are formed by conjugative plasmids or transposons, this also allows nonconjugative transposons and plasmids to be transferred to another bacterium. Transformation occurs when bacteria take up extracellular DNA and incorporate it into their genomes. Typically, this occurs when one bacteria lyses, or splits open, releasing its genetic contents, and then another bacteria comes by and acquires it. Bacteria able to bind large amounts of DNA are termed “competent”. Competency is a state of increased cell wall and cell membrane permeability that allows cells to uptake DNA. Many bacteria are naturally competent, and so actively bind environmental DNA. After transport into their cytoplasm, the bacterium can incorporate the new DNA into its genome through the process of “recombination”. Recombination is the rearrangement of donor and recipient genomes into new, hybrid genomes. This can result in new phenotypes – for example, the bacteria can acquire pathogenicity or antibiotic resistance. Some competent bacteria actually kill noncompetent bacteria to release DNA for transformation! Transduction occurs when DNA is transferred from one cell to another by a bacteriophage – a type of virus that infects bacteria. Viruses cannot replicate on their own – they are obligate parasites that rely on host machinery. Many bacteriophages can switch between a state of lysogeny and a lytic cycle. When the bacteriophage is in a state of lysogeny, the virus combines its genome with the bacterial chromosome. The viral genome hangs out there for many generations as the bacterium replicates. When induction occurs, the virus switches to the lytic cycle. The cell becomes a virus-producing factory until it gets so full of virus that it lyses, or bursts open, releasing virus particles into its surroundings. There are two kinds of transduction – generalized and specialized. During generalized transduction, the phage capsid accidentally assembles around a fragment of bacterial DNA or a plasmid. When the assembled viral particle infects a new bacterium, it injects its previous host’s DNA into its new host. Specialized transduction occurs during the lysogenic life cycle of the virus. Incorrect excision of DNA during induction results in a fragment of bacterial DNA being picked up instead of a part of the viral genome, which then stays in the bacterial nucleoid. Now, the bacterial DNA replicates as part of the bacteriophage genome, is packaged into phage capsids, and is injected into new bacteria. Horizontal gene transfer, along with mutations, allows bacteria to achieve genetic diversity. Mutation is a slow process, and most mutations are harmful or neutral, not beneficial to the bacterium. Meanwhile, horizontal gene transfer is a rapid way to acquire large chunks of DNA from another bacterium all at once.

[yoaimou]

Imagine being a bacteria and watching this, then your bacterial parents walk in 😭

[longhuynh-cmdt]

bro, who let the animator cook? 💀
them bacterias look naughty asl

[ericelvin]

I m a medical student and for a long time I have been facing a lot of confusion in this topics. Thanks for helping . Good day👍

[mathumeethakk]

Understanding is a hilarious task we students face. Such illustrations make me admire the way you made this concept to reach the people and also your creativity

[DeadInside-rywq]

0:05 h or l gt
0:18 3 ctt
0:28 t and t 2b
0:35 c port
0:48 transposomes
Cell contact. 1:06
1:20 g- fp . Conjugation pilus
All will get

2:15 TRANSFORMATION
2:31 envio
2:44 competent
3:08 recombination
3:18 new phe
3:30 TRANSDUCTION
3:37 V CABT REPLICATE
3:49 lysogny
The switch to lytic
4:20 two types
G and s
4:30 g
4:37 s. Lysogenic.

Genetic diversity 5:01
Hgt and and mutation to get genetic diversity.

5:22 slow
5:27 hgt get otehr bac dna
5:44 genome size constant


THANK YOU FOR THE VIDEO

[Kayla-wqpf]

nursing student here! was struggling to understand this in my microbiology class. i have an exam tomorrow morning and will definitely be thinking back on this video, thank you!!!

[goldie]

Oh. My. Goodness. This is the best video ever 😍 I am so glad I found your channel <3

[Raamy]

CONJUGATION 00:57
TRANSFORMATION 02:13
TRANSDUCTION 03:32

[medialunasx]

Im a third year med student who is taking Microbiology, thank god for this video or else I would have taken hours to truly comprehend what I was reading

[imhomewiththemilk]

this was the best video thank you so much!!! very entertaining and fun, but i also learned ALOT that my microbiology book didnt teach!!

[stephanieorrego-lopez]

i'm living for the visuals🙏🏼💗 thank you !

[JrysonTheEditor]

This is the scariest video I have seen in awhile

[hannahcloots]

i love your video, it's very helpful and a good animation to understand the topic better!

[simplysteph..]

watching this as a last minute savior for my exam tmr

[feliciapabi]

Very educative, and I understood everything.😅Very great full 😅

[Inxx]

The animation is great, thanks for making!

[mollyngonga]

I love this, I understand because I could relate to the pictures it made my learning process more fun..Kindly male more of these

[amesteshearn]

Nice work! Using for my General Microbiology class - videos like this that have great fun, illustrations and are solid info really add to class discussions. Also, appreciate the mutation aspect - perfectly aligns with Am. Soc.Micro learning objectives. Thank you!

[selamawittaye]

Ufff you saved me ! Thank you very much!

[laman]

1:29 yo, that purple bacterium was enjoying that way too much!