Determining MW of proteins on SDS-PAGE

[barbarellagyigrejalde]

Thank you so much for this. Extremely helpful for writing my lab report.

[nikolaspais]

thank you so much, i study biochemistry and this video was really helpful!

[MonaraKaelle]

Thank you for sharing it.
To make a scatter plot in this case, should I always transform to log?

[pauliaa]

Hi, so maybe a stupid question, but where do I get the migration distance from? Do I just have to measure it ? Thanks.

[danaechatziandreou]

Isn't it more accurate, if the plot is with Rf and log10(kDa), rather than the distance and log10(kDa)?
Thanks for the video

[qamarhennawi]

thanks for sharing, I have a question is that some people are calculating according to the migrate distance over the total distance, which way is the correct way? Thanks

[fardinmiah]

if i use Rf can I use the same equation and method?

[_han_da__]

Is the molecular weight depending of one protein or more?

[pollyannagomes]

Hey, your video is the best but how can I calculate the cm of the bands? I need to take a scale to do this? Please help me lol

[earthstar]

Hello, I have a doubt, when you try to find a target gene with a respective number of base pairs for example 300bp and when you do the mathematical calculation that fragment or band turns out that it has more base pairs or ranges from 380 BP, in this case, did you rule out that it's the target gene or is still the target gene?
Thanks for the information!

[solakhalid]

Plaese. I need the word file.. Can you send me?

[gauravresearch]

Great video. Thanks. Only thing that I also like to understand is how you can measure distance in cm using excel. I know other software's for that.

[hermainrais]

Hey how are u can u please help me in my assignment question