SDS-PAGE explained - Protein Separation Technique

Hey Friends,

SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) is used to separate proteins in a sample according to their molecular weight.
SDS binds to linearised proteins and masks the native charge. 1.4 g SDS bind per 1g protein, so that all proteins have a nearly similar mass-to-charge ratio.

After an SDS-PAGE, the gel is frequently used for Western Blot analysis to confirm the presence of a specific protein of interest.

Western Blot:

Here is a helpful explanation on SDS-PAGE:

Thanks & Bye
Henrik

[amrobay]

wow, after each video, i ask myself "why professors can not explain it that simply", thanks a lot!

[rahman]

at this point, i think the prof don't understand the process, if you can't explain it this simply as in this video, then at least give us link to videos that explains it better to make our life easier

[kimbokjoo]

I watch all the ads for Henrik. He’s been very helpful.

[MattMatek]

this is the best SDS-Page explanation on the entire internet, thank you

[isadorah]

I want to become a teacher which explains clear and easy like u!!!

[chantikaanindhi]

thank you so much, it's really helpful, i'm cryingg

[SaraMonicaLeonUlloa]

Excellent material! This is a great job explaining and illustrating the topic. Very clear information.

[dermdoc]

bro this video is so good and so easy to understand thanks a ton dude

[fordmustang]

Thank you very much you just save my marks for viva

[victoriafroes]

Perfect! Thank you so much. This help me a lot 😁

[hnrasouli]

Such a wow video should be appreciated. Thanks for sharing this video

[wissammukaddam]

What a brilliantly concise explanation, thank you.

[galaxyeyes]

Thanks for making these nice videos. They’re very clear and the diagrams/animations are really helpful

[refad]

Very accurate and clear explanation. Thank you

[TensonNthambo-px]

This is nice, am able to understand the process now

[lotti]

thank you so much, you helped me a lot with my lab report!!

[shyamasham]

Thank you so much for explaining it so simply 🙏🏻💛

[philbertnshimiyimana]

I like this tutorial much. Very hepful in my current research project on p38 protein kinase

[graemelaubach]

You make the most informative and easy to understand videos. Thank you :D

[razasyed]

amazing video and explanation, thanks hoss