Single spore dilution plating for isolating monokaryons.

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How to plate spore dilutions for single spore (haploid) isolation. Once they germinate, monokaryotic (hopefully) colonies will be checked for the absence of clamp connections and subcultured. They'll then be clear for use in mating experiments.

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  1. Edward Grand
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You skipped the serial part and went straight to plate! I'm gonna try it.

mycoGeeky
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It's that simple? 🤯 Woah that's cool

Mind
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what are those glass thin nosed droppers called?

mycoGeeky
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Got a part 2? How do you know for sure you only got a single spore? I suppose you need a microscope, or when it grows out can you distinctly tell. I know you did a big serial dillution, just trying to better understand how breeding is done and you look to know a few things for sure.

coloradodirtbike
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Reverse osmosis water ok? Should I adjust the PH? My RO is ph5.9 with a TDS of 0 .
I have seen plants that like the gray water from my RO unit and wondered about mushrooms what water they like best?

gangsterbuilder