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Ghetto Serial Spore Dilution and Plating (from swab) for Monokaryons
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0:16:30
Ghetto Serial Spore Dilution and Plating (from swab) for Monokaryons
0:11:23
Ghetto Spore Dilution Method (from swab)
0:28:23
Verifying Serial Dilutions with the Microscope: Multi-well Breeding
0:09:03
Isolating haploid, monokaryon, single basidiospore isolates (SBIs) from a serial dilution plate.
0:13:40
Picking/isolating monokaryons (haploids) from swab serial dilution plates for mating/breeding.
0:11:56
Double swabbing, ghetto swabbing, grab and drag, microscopy, cross verification, fingerprinting.
0:21:48
Breeding Mushrooms Part 1: Serial Dilutions and Isolating Haploids
0:05:20
Isolating monokaryons (haploid) cultures for mating/breeding - Spore drop method on a tilted plate.
0:11:39
Monokaryons (haploids for mating/breeding) from a swab. Take 2!
0:07:01
Isolating and selecting a monoculture for your growing conditions, when to swab, and how to use it.
0:11:54
Monokaryon (haploid gametes) macromorphology, tomentose, rhizomorphic, mating, breeding, isolation.
0:13:19
Monokaryons from a spore print. Maybe even some MSS and dikaryons from the same procedure.
0:07:24
How to use swabs. Getting a clean dikaryon, monokaryon, and maybe even a phenohunt!
0:11:54
Mushroom Breeding - spores, monokaryons, anastomosis, and somatogamy (dikaryon formation).
0:15:03
Managing monokaryons for crossing/breeding/mating experiments - workflow, labelling, and sanity.
0:09:55
Swabs 101 - How to efficiently and effectively use them to get a dikaryon and maybe even some monos!
0:10:49
What is a monoculture, haploid, spore, multispore, monokaryon, dikaryon, swab, LC, MSS, T0, isolate?
0:13:38
Swab plating technique to get a dikaryon, possibly monokaryons and preserve the swab for future use.
0:08:10
Preparing a slide to check for monokaryons with the microscope.
0:02:49
Confirming an haploid, monokaryotic culture to be used for hybrids. Success!
0:01:00
Easy Spore Swab Techniques
0:05:53
Pulling/Isolating Toque monokaryons from a 'grab and drag' plate. Note: use dark-colored p...
0:00:15
B+ Liquid culture
0:15:37
Monokaryon mating/breeding/crossing (mon-mon) set-up and procedure for producing F1 progeny.
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