Isolating a single spore under the microscope

This video shows the breakdown of how I isolate a single spore from a wet slide using a pipette. Enjoy and MUSHLOVE

Amscope link
AmScope - 40X-2500X LED Digital Binocular Compound Microscope

🔬🧪🧫 on 1 Mushroom EXPERIENCE:

@freshfromthefarmfungi

[robbyhanlon]

Gary, I’m pretty sure you are the only dude on YouTube doing this kind of in depth content. Believe me, I’ve searched. We appreciate it. I am wanting to understand the process of breeding more and there just isnt a lot of content out there.

[InconspicuousOwl]

This is the one I've been waiting for! Cx

[AaronAlso]

Them is some skills, my hands just aren't steady enough.

[HChandler]

Now I gotta figure how to justify buying a microscope to my wife 🤣 she'll understand 🫡

[tonystewart]

Thank you for another great informative video Gary.

[moisesaramos]

Gary is finally showing his gangster stripes. Digging the metamorphosis, also I knew he wasn’t just some gourmet cultivator.

[JUSTINOURSE]

Thank you so much for showing how to narrow a Pipette.
I didn't know that this was possible till now.

[amanitaocreata]

This is why you're becoming my favorite to learn from

[coloradodirtbike]

Such highly valued info that very few people ever show or even know how. You are a master Gary and your expertise and knowledge really is endless. Thank you for taking the time to teach others.

[sporgymushrooms]

Great video Gary! This makes me wish someone would make an iso printer that could print single spores perfectly spaced out on agar plates. Would love to see a video about strengthening genetics and principles of inheritance.

[marlinmixon]

I watched your video where you divide a spore solution multiple times until you get a low enough spore concentration to easily isolate single spores. I thought a bit and you might be able to do the same thing with sticky tape. Make a spore print on the sticky side of sticky tape, then line up a bunch of sticky tapes in a row and touch the spore print side to a fresh piece of tape sticky side to sticky side and pull it apart which probably removes half of the spores then grab a fresh piece of sticky tape and touch the last piece of tape together with the new piece. Eventually you get down to single spores. Not sure if a single spore on a piece of sticky tape is useful but was an interesting thought exercise.

[syberphish]

Hey Gary, what's a good reference for identification based on size and other spore attributes?

[benjamindejonge]

Omg when you think Einstein figured it all out, somebody comes and says hold my beer

[ErikPfau]

After isolating a haploid, and growing it out on agar, and combining it with another haploid, will the entire monokaryon become a dikaryon? Or only where they fuse together? Can you make a transfer from the farthest side and it still be monokariotic or does information travel through and know it has mated?

[evilanana]

Bro before the video even started I saw the spore print in the petri dish and I woke up my daughter saying "that's effing genius" lol.

[ScottWConvid]

What is the advantage of isolating single spores over allowing spores to randomly mate on a media plate or other nutritious matter? Is there a way to determine which spores will have better genetics?

[Milkex]

MAP gas is sold in yellow, propane tanks are blue

[kainophile]

I was wondering if anybody went through this kind of trouble to get haploid spores. Do you make a practice of doing this?

[nubletten]

First time i hear about this is from you.
Imagining a cross between cordycep militaris and lions mane that grow very well in wood and eat pests.
Also it would look like a cordycep stalk with lions mane cap or reverse :))
This have crazy potential imo.

[revolioclockbergjr]

Can this be achieved with a hypodermic needle?