Fungi transfer on Agar (subculture)

This is how to transfer fungi to new PDA (subculture) by using surgical blade

[leahe]

Thanks! I appreciate the demonstration. Some of us are visual learners :)

[viviananthony]

I did this yesterday but i used a loop instead, hope thats ok?

[CuriousFunguy]

Why flame in front of laminar flow ? this would disrupt laminar flow and seems counter productive but I'm not sure.

[kimyeontan]

is it ok to use a sterile loop to transfer a mycelium?

[purwobiology]

Can I know how to isolate fungi, such as Aspergillus niger from environment?

[aliaaebidy]

Why the plate was black ? It's PDA?

[motivatemee.]

Why did I expect you to cut it like a cake? You disappointed me 😅

[Yui_Fukushima]

My agar is kinda too wet, i wonder if it will grow on it :((

[teresashinkansen]

In our lab we were forbidden from doing work with filamentous fungi on the laminar flow hoods because that could contaminate the hood filter and when dealing with pathogens it can be dangerous. It was all done on tube slants and besides a large burner. Also why burn the scalpel, wasn't it sterile already?

[afrosch]

Why do you heat sterilize a sterile blade? 😂

[whoisit]

After subculture, how long does it have to wait to get fully well cultured?

[olusolaakinade]

What you cut out of the agar, is it what is called a mycelial disc?

[afiqaizat]

Can i know why u dont use streak plate technique?

[CFOSolnetInfo]

Can we transfer bacterial agar plugs just like this to plate that has lawn of indicator bacteria to screen bacteria for bioactive compounds/antibiotics?

[lalicejenn]

After cutting it and then placing it in a new place, is it not coming back? Is that okay? Sorry, my English is bad

[BrutallyHonest-vqlh]

Not sure why YouTube recommend this shit to me, and didn't get what I just saw and don't wanna know it.

[hodamaher]

Please how to isolate and culture Aspergillus oryzea????