How to determine the Protein Concentration with the Bradford Assay

Hey Friends,
protein quantification techniques are used to determine the total concentration of protein in a solution. One among many colorimetric assays for this is the Bradford Assay developed by Marion Bradford. The assay is easy to perform and delivers results in a short time. The Bradford test is based on Coomassie Brilliant Blue G250, a dye which binds to proteins in an acidic environment.

References:

Thanks!
Henrik

Time Codes:
0:00 Introduction
1:04 Coomassie Brilliant Blue
1:44 Protein Concentration Determination
2:58 Advantages/Disadvantages
4:02 Outro

[lilachgavish]

Thank you very much. I send this clear and simple explanation to all my students.

[mak]

Huge compliment for your videos Henrik!
I would like to see some videos about RIP (RNA IP) and CLIP.

[migueltrujillo]

a great video!!!!
"keep it simple" is the rule
:)

[나은랩]

감사합니다! MO를 이용해서 색이 바뀌는 이유도 설명해주셨음 더 좋았을 것 같네요.

[mansisahare]

It's really easy and understanding video

[abdulazizabdunasimov]

I bet you're a german speaker. Thanks for the video

[javieramacarenao.c]

Thank you so much for this, it was very useful <3

[samanthasaboia]

Thank youuu so much for this 🙏🏼🙏🏼🙏🏼💙💙💙💙💙

[Luke]

Hello. In so many articles i read that coomassie blue transfers a free electron to the protein and this causes denaturation. Can anyone pls explain where this free electron comes from?

[yurimasier]

Can i ask why the SO3- is not protonated in acidic enviroment?

[joaopauloferreirarodrigues]

I can buy the Bradford detergent compatible

[crimetax]

bruder mach ma auf deutsch in kann kein englisch