Designing cloning primers for classical (restriction) cloning

Video use for teaching on module 500709 Cellular Regulation and Biotechnology at the University of Hull

[ForestBird]

Thank you so much for making this! I've spent hours trying to understand how to do this and you cleared up all my confusion in just 20 minutes.

[eyosightyekarawork]

3 weeks of my lab explained In 20 you so much for this resources

[caleblee]

I have an exam in 3 hours and this just saved my grade. Thank you Katharine!

[arabicanimation-]

Im a Student in Germany.. your video ist more than excelent.. thank you a lot ..

[alicebrown]

thank you so much！Your explanation about the stop codon really helps me a lot with my assignment.❤

[ChrisLainas]

Amazing video, helped tons with my synthetic bio assignment! Many thanks from Canada!

[jamesc]

Thank you so much! Your explanation was very thoughtful and straightforward–I appreciate it!!

[shaktiprakash]

thanks mam, you save my money and time. God bless you. No one had explain the STOP CODON properly

[separase]

Thanks for the very good explanation! One question. XhoI restriction site is 5’-CTCGAG-3’. However, in the reverse primer example in this video, the added XhoI RE site is written as 5’-GAGCTC-3’. Is the nucleotide sequence of the XhoI in the reverse primer correctly written? Thank you!

[zahrahammad]

You are exceptional, plz also make videos on homologous recombination, expression plasmid and chromosomal integration too

[AmruMagdy]

you cleared up all my confusion in just 20 minutes

[Huy-tumx]

Thank you for sharing! Very informative! You're such an effective educator!

[shraddhakasoundhan]

I was so confused earlier... thankyou for resolving it..

[felipecwouters]

That was a great video! Simple and very well explained. Thanks a lot!

[আমারশখ-ভ৯হ]

don't the two primers add to different stands of the target dna? then how did both the primers end up in the same dna in the second step in the pcr?

[triyudaniyudanimardiningra]

Prof Hubbard, thanks a lot for your excellent video. very well explained.

[mdzakariamorshed]

please make a video about the primer design regarding golden gate cloning primer design

[GeorgeCronigen]

I cannot understand since there are restrictions sites on the plasmid which will be cunt the restriction nucleases then why do I need to have restriction site on my amplified gene ???

[chandramoulikaponnam]

how to design primers if we want to add a tag to protein?

[ookamit]

Really a simple and good explanation. Great job 👍