How to design Primers ?

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#multiplefragments #primerdesign #NEBuilder
In this video, I have used an online NEBuilder tool, which can be used to design primers for multiple gene fragments. With the help of Fragment Assembly Using NEBuilder HiFi DNA Assembly, see how simple it really is to clone lengthy gene in multiple fragments.
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MashaAllah zabardast informative video for researchers

samkhan
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is there any software sir where we can upload our query sequence to be cloned and as well as the vector in which the query will be cloned so that we can get the repective primers for that query gene alongwith in the output mentioning the restriction sites incorporated in primers

sreeram