Overview of Traditional Cloning

0:00 - History of cloning
0:29 - Step 1: Identifying restriction sites
0:57 - Step 2: RE Digestion
1:27 - Step 3: Dephosphorylation and other end modifications
1:55 - Step 4: Ligation
2:00 - Step 5: Transformation
2:12 - Step 6: Screen for desired clone
2:17 - Reagents for Fast cloning

[deetijyothi]

Thanks for ur vedios. I am a non biology student and doing my PhD on Protein X-ray crystallography. Ur vedios are really understandable and helpful for hands on also. If possible please make some vedio on basics and mechanisms of protein expression and purification related. Thank you