A Detailed Look at Gibson Assembly

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Delve deeper into #GibsonAssembly with this detailed look. Learn about linearizing your vector, designing PCR primers, and performing the Gibson Assembly reaction with this in-depth video.
IN THIS VIDEO:
00:00
00:55 Step 1: Linearize Your Vector
01:45 Step 2: Design PCR Primers
03:25 Step 3: Gibson Assembly Reaction

  1. SnapGene
  2. SnapGene
  3. Gibson Assembly
  4. Molecular cloning
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Комментарии
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I've watched so many Gibson assembly vids and this is the first one that I understood. Thank you!

HarveyDub
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Thank you so much! I can understand what I try to read on the internet for like 3 hours in less than 3 minutes.

theanonymous
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How do you control how many bases are chewed by T5 exonuclease? I don´t understand why the exonuclease doesn't digest the whole strand

omarsanchezjimenez
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How do you justify primer design for short (0.1-0.5 kb) and long inserts (8-10 kb)?
And what about medium inserts of 0.5-8 kb?

SonVu-juko
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Thank you but I still don't understand how to create the overhangs on multiple fragments using snapgene.

rukayatjimoh