Dot blot technique | principle , procedure and application |

Definition : Non-electrophoresed biomolecular samples (DNA, RNA, protein) are directly blotted on a nitrocellulose or nylon membrane as dots for identification of nucleis acid or protein. 

Advantage:

1) Simple process of biomolecular identification because no electrophoresis is required and direct blotting on membrane .

2) sample from different source can be tested in a single run . 

Disadvantage: 

No information about the size of the target molecule .

Procedure: 

1) for nucleic acid 

         a) extraction of DNA or RNA

         b) add to membrane 

         c) for DNA, denaturation 

         d) immobilization

         e) hybridization

         f) autoradiography

2) for protein 

         a) protein added to membrane 

         b) blocking 

         c) peimary and secondary antibody

         e) detection 

3) application: 

         a) identification of transgenic individual 

         b) estimation of protein concentration.

[anmariyavarghese]

Nice presentation. It's my seminar topic. It help me a lot. Thank you

[watanmdc]

very simple and understandable, thank you

[YoyoBear]

thank you for posting this video! it helped me so much

[sharathmb]

Thanks bro ... I understood that completely 😘😍

[RahulKumar-mmvm]

Dot blot technique can be used to identify highly degraded male dna sequence below 50 base pair ?

[forwardfamilyfarmer]

respected sir do you give a reference in which shown that Dot blotting technique used to identify the specific sequence of DNA.

[sanchisingh]

Thank u sir.... tomorrow is exam quite helpful 😅

[befashionable]

U should make more video
U r osum sir

[nabamitanandi]

good content, little improvement required in the way of explaining, keep it up sir