Amyloid Oligomer Preparation and Assessment by Dot Blotting Analysis | Protocol Preview

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis - a 2 minute Preview of the Experimental Protocol

Huang Chunhui, Xu Dilin, Zhang Ke, Shentu Jieyi, Yan Sicheng, Wu Dapeng, Wang Qinwen, Cui Wei
Ningbo University, Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine; Ningbo University, Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center; Ningbo University, School of Materials Science and Chemical Engineering;

This protocol describes how to prepare Aβ oligomers from a synthetic peptide in vitro and to evaluate relative amounts of Aβ oligomer by a dot blotting analysis.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.