Southern Blot Method - Animated Video

I make animations in biology with PowerPoint, and this animation video is about southern blot method. Which combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.

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[priyanshi]

Im just dropping a comment to let everyone know these 10 mins were totally worth it! Amazing job!!🥰

[dr.aldosppd]

Amazing video! I'm sharing your video to my fellow biotechnologist alumni. One of them is now a vice dean in the biotech faculty where we studied together. I hope this will help your channel grow 🙏🏼

[meriem]

Thank you for all the effort you put into each video. It is not easy what you do 👏👏👏 so thanks for doing it and letting us learn what seems to be complicated before.

[alix]

Super impressive animations! Thank you!

Edit: Saw that they were made in Powerpoint! That's even more impressive!

[Alvaresd]

Nem sei como agradecer a existência desse tipo de vídeo. Vocês são incríveis ❤

[adisenglish]

art, knowledge, and wisdom. All in this video

[chulaekanayaka]

Simply: the best of 20ish videos I watched on this unit

[dmbms]

Thank you for all the effort, Amazing video

[drishtantsen]

Brilliant animations, perfectly condensed and presented

[somchart]

Best explanation of not only southern blot, but the gell electrophoresis ☹️💗👏 thank you very much .. i’ve always wanted to understand it

[oliviamotivation]

Astounding video! It's very helpful to understand the concept easily, especially in online class . Thank you so much sir.🙏 👏🏻👏🏻👏🏻

[lidijadomitrovic]

This video is made so systematically and shows many important methods concisely in one place. Thank you for that. Also, I think you could explane things better concerning gel electrophoresis. Shorter DNAs move faster and migrate farther than longer ones because shorter migrate more easily through the gel

[dominickbo]

super amazing! i really appreciate your video for my upcoming exam! thanks for the phenomenal explanation!

[bilaselsel]

Thank you so much professor . Now and thanks to you animation I understood this method very well .

[meenakshypradosh]

It's a nice video and is very helpful❤

[Mobilename]

Very helpful, now I can attend any question on this topic

[dishninuwandika]

Thank you so much for this vedio.Great job. Very supportive for our studies.Thank again all of you

[aritrikamajumdar]

Amazing video. Extremely simple and easy to understand. Thank You so much for making such a good and informative video on a complex topic in such a simple manner.

[fahimalgulib]

Impressive!! This is a very great job and im very much helped by you. thank you for making this

[RohitPant]

In a neutral pH range, pH 5 to 9, DNA molecules are quite stable. However, if the pH becomes too acidic or alkaline, DNA molecules are prone to destabilization.

At pH 5 or lower, DNA is liable to depurination (i.e. the loss of purine bases from DNA). The phosphodiester bonds of DNA break which causes the base pairs of DNA to break off.

At pH 9 or higher, DNA is susceptible to alkaline denaturation due to the abundance of hydroxide ions. These negatively-charged ions remove hydrogen ions from the base pairs of DNA, thereby breaking the hydrogen bonds between and causing the DNA strands to denature. While less damaging in comparison to acidic pH levels, high alkaline pH levels can still result in depurination.