Dot Blot Technique ll Dot blot protocol ll Xgene and proteinX

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Dot Blot Technique ll Dot Blot protocol ll Xgene and proteinX

Here I showed how to perform dot blot technique. dot blot protocol. It is a technique to identify and detect antigens and antibodies in the sample.

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I have a question. In the case of antigen in the sample where you first added the antibody and it dried up, How do you achieve a control for that. Because since the antibody is loaded on to the membrane first. Won’t it bind the secondary antibody regardless of the binding to antigen. Also what’s the ratio of background when done ??

henryuciyke