CRISPR-Cas Genome Editing in Mammalian Cell Lines | Protocol Preview

Genome Editing in Mammalian Cell Lines using CRISPR-Cas - a 2 minute Preview of the Experimental Protocol

Kaiwen Ivy Liu, Norfala-Aliah Binte Sutrisnoh, Yuanming Wang, Meng How Tan
Agency for Science Technology and Research, Genome Institute of Singapore; Nanyang Technological University, School of Chemical and Biomedical Engineering;

CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing efficiency.

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