CRISPR Cas9 : How CRISPR can be performed in the lab ?

This video describes the detailed protocol of CRISPR Cas9

[jamesdillard]

Great video, thanks for sharing. One of the few I've seen about the actual workflow of CRISPR edits.

[divyathiagarajan]

A very informative Video. Thank you for clearly explaining, in a easily understandable manner for a beginner like me.

[williamwallace]

Nice demonstration. My question is why not label every plasmid (sgRNA, Cas9)) with different fluorescent protein (green yellow, red, etc) and sort the cells with two fluorescent proteins and expand from there ? This saves time and effort ! Also you can use antibiotic resistance.

[mohammadmubarak]

Great informative video!! made it very simple and easy to digest.. cheers!

[pritomc]

Many thanks for your video. Could you please share with us which tools did you use to make this animation?

[bhagwatjadhav]

Can you share a paper or source for transcriptional activation repression of RNA by crispr cas9.

[Ahmad-yvmo]

what are the three ways to upregulate the expression of a gene in arabidopsis using the CRISPR/Case9 method...pease sir help me in this

[teensharksstartupschool]

at around 9:48 you said it is tedious. How long does it take to run PCR on all three "target colonies". What part is the most repetitious and tedious?

[Lily-]

I have few question
How far we are from these technologies, i mean precision medicine?
Who will able to afford it rich or poor?
The way climate changes occuring so fast will it be able to inbuilt these precision medicine in particular individual?
To make precision medicine how much time is needed for one person, average time?

[latinadna]

Hey Arpan! I really like your graphics! Where do you find them? Especially the ones in slides 4-6?

[monerjanala]

At what stage of cell can this Technology be used?

[LAmourDeViva]

So what are the long term effects....will it reverse, mutated or evolve in the future?

[catarinabarbosa]

Hi!! Could this technique be possible to use in photoreceptor cones?

[Priyuge]

When we expect this treatment for muscular dystrophy treatment

[priyangshubagchi]

Dada I'm from, kolkata and I am in class 10th, I'm looking forward to start a startup based on Crispr... So I wanted to ask you. Is this idea good??

[dasdasdsdasdasdasd]

I can't understand his accent. ;)

[skittle]

can i become spider man by genetically modify

[HUMAIRAKHAN_]

Jesus is the prophet of God not son of god

[SherriP]

Jesus is the son of God who died and rose three days later so you can be free forgiven healed delivered saved baptized and so much more. Believe in Him as the son of God and you are saved eternally and will live eternally. He's awesome and wants us knowing Him and spending time with Him. Ask anything in Jesus name and He will do it.