Pooled CRISPR-Genetic Screens in Mammalian Cells | Protocol Preview

Pooled CRISPR-Based Genetic Screens in Mammalian Cells - a 2 minute Preview of the Experimental Protocol

Katherine Chan, Amy Hin Yan Tong, Kevin R Brown, Patricia Mero, Jason Moffat
University of Toronto, Donnelly Centre; University of Toronto, Department of Molecular Genetics; University of Toronto, Institute for Biomaterials and Biomedical Engineering;

CRISPR-Cas9 technology provides an efficient method to precisely edit the mammalian genome in any cell type and represents a novel means to perform genome-wide genetic screens. A detailed protocol discussing the steps required for the successful performance of pooled genome-wide CRISPR-Cas9 screens is provided here.

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