Introduction to HPLC - Lecture 6: Band Broadening in HPLC

A lecture series on HPLC covering everything from theory and background to practical trouble shooting. Lecture 6 provides a look and band broadening, a common problem encountered when using HPLC. We discuss what it is, the Van Deemter equation and the factors at play including Multipath (Eddy) diffusion, longitudinal diffusion, and mass transfer.

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[fudge]

I watched your series back to back in preparation for a job interview. Thank you so much for the great videos!

[GiorgosGkousios]

Amazing series, unfortunately those vids werent made in 2019 and lost tons of hours reading for HPLC! Great channel, great content! Thank you!

[faridehkadkhodaei]

Just finished this video and I want to say thank you for this course and all other videos in your channel. I really appreciate it. I was looking for a video on GC, but I could not find a course on the fundamentals of gas chromatography in your channel. It would be great, If you could please teach us this topic as well. Thanks again.

[PrabhuDHASAIYAN]

Could you please uploads a video on how to use HPLC for determining concentration.

[mhamado.abdullah]

Wow, amaizing explanation, thank you.

[dimdimbramantyo]

Great lecture, thank you so much for this. I'm learning HPLC for my thesis, and it isn't as easy as it looks haha. I hope you can make more lecture videos about problems in HPLC such as mobile phase and stationary phase selection, pH and buffer usage, etc.

[maryyoussef]

Thanks so much, it is really helpful series and very informative
Can you please share one about LCMS !?

[oleglookin]

Thank you very much for this course! I have started my learning of HPLC a couple of days before and your lectures advanced me in the understanding very greatly. Btw, if one uses not columns but capillaries, does the factor A in Van Deemter equation still play? Thanks!

[charmming]

Thank you very much . are there another lecture ??