HPLC Chromatography Basics Explained

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All you need to know about the HPLC Chromatography!

HPLC(High-performance liquid chromatography) also referred as high-pressure liquid chromatography, is a technique used in analytical labs to separate, identify, and quantify each component in a mixture.HPLC separates and purifies compounds according to their polarity.

I have tried to simplify the whole process of HPLC Chromatography and explain in simple terms. Let’s look at the main components involved in HPLC in this video.

Later in this video, I discussed about Normal Phase Chromatography, Reverse Phase Chromatography and their comparison and differences.

Watch this video for more details.

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If you have any doubts: Please leave a comment in the comments section below.

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[shoaibsheikh]

I did B.Pharmacy but no body taught me like this during my college years. It was very difficult to understand by reading only theory. You have great knowledge mam and the way you explain is excellent. I am subscribing your channel now. I really appreciate your hard work. Thank you.

[misbahmalik]

honestly no one can teach better than you. How perfectly you explain each n everything. Truly helpful and impressive

[saydfouz]

thanks a lot for making us aware about the basics of HPLC. Before meeting you, I never thought that a person could be a teacher, support, mentor and philosopher all in one! Thank you for all your hard work and dedication!

[SheikhLateef]

I m watching this video again after 3 yrs. And Thanks mam. i wish i could attend ur class in front of uh ..and. i deeply love with ur style of teaching

[sushantpople]

I was very worried about my MSc Bioanalytical Science's, due to online studies i never understood the any chromatography techniques, but today only i found this channel i saw hplc video and now i am not having doubt..i will going to watch more videos, Requesting to please add Bioanalytical related topics..thankx

[princesharma]

Most easiest and valuable information about HPLC on YouTube... respect to you mam 👏🏻👏🏻

[harleenkaur]

Thank you ma'am Tommorow is my environmental chemistry paper and I am going to write the same watching chromatography videos and this one I feel it's simpler now

[sushreepriyadarshanimahapa]

Tq mam ...tq so much.because of uh i can my +3 final year DSE-1 papper successfully 😍love from Odisha 😊

[syedmehtabgilani]

I, m Chemist in Pharmaceutical Industry & perform HPLC. Your Lecture is very helpful 4 me

[Martis]

Aisi teacher kidhar milti hain... Humare Clg main to sab English ratke chale jate hain..

Best explanation👏🏻

[PrinciThapak_scienceelite]

Your lectures helped a lot in my studies... Please keep making these videos...😊👍

[muhammadnaseer]

I'm from Pakistan your lectures are very helpful thanks Madame.

[bhawnaa_]

Absolutely amazing teaching. Very proper point to point knowledge. So much helpful and accurate. ❤️❤️❤️

[anjalisolanki]

Thank you mam for such a nice explanation, , , i understand every single line you tought. 😊

[amitkboora]

First time i have seen huge likes on scientific instrument videos…
Keep on

[nirajsalvi]

Thank mam
U are great
I want to know about more vedio
Like kf, gc, HPLC, and their tribal shooting
In industrial use

[sailajamaddali]

Thank you very much for your presentation. Would like to know about the buffer you mentioned to equilibrate before the sample run (getting the base line). can you explain more about the equilibration and the buffers to use thank you!

[varsharani]

Thank you ma'am for explaining in such an amazing way, this clear all my doubts.!! 💓

[--AgamdeepSingh]

mam u are nirraa ishq teacher what a beautiful explanation of hplc..kudos to your hardwork 💯

[gururaj]

Mam iam from andrapradesh still iam able to understand ur simple understandable hindhi tq so much