Introduction to HPLC - Lecture 3: Reverse Phase HPLC

A lecture series on HPLC covering everything from theory and background to practical trouble shooting. Lecture 3 provides an overview of reverse phase HPLC in which hydrophobic columns and polar mobile phases are used for separation and retention time analysis.

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[shauryatiwari]

This is the most thorough HPLC series I found. Brilliant work. Thank you good person.

[derekedington]

thanks for the hplc vids. in biotech program in dayton and you do a great job explaining everything.

[EIsuruPriyaranga]

Thank you so much! You saved me and a great starter for my new PhD project!

[ChoocoCaramell]

Thanks for the effort in putting this material on youtube, I've been searching more of all of this topic and I don't find more clear material than this!! thanks a lot :-)

[YuvrajYadav-qprq]

Thank you man for this hope you're doing well again thanks a lot.its overpwoered content

[akbarbahrami]

Very impressive way of explaining materials. Loved that!

[soheylasiavoshi]

Thank you Sir. It was so practical for me🌸

[azarkhany]

thanks and can't wait for the next one!

[lydiaamelia]

I really love how you explain all of this, its so clear and i could understand it easily, thank you so much for making this lecture 🙏☺️

[pharmabot]

I love your lectures! - A small tip: PLEASE start using a wacom tablet instead of a mouse for writing on the screen

[stijnstrotmann]

These lectures help me out so much!! Thanks a lot! <3 Thinking about buying your beginners guide too. Unless its your video's in text format.. I allready made a lot of notes. ;)

[Swashbucklebuckle]

Up to this episode, this series has been extremely insightful and helpful! I think this knowledge will help me very much in my upcoming job interview(s).

[seyedmohammadseyedkhademi]

Thanks for this video, but I have one question at the time 43:13 of this video. you told that H2O/MeOH (in comparison to CH3CN/THF) has a higher Rt or longer spread. While in Revers Phase, the Stationary phase is Hydrophobic, and the more polar mobile phase should elute faster. Or I, m wrong? please explain a little more about this. Thanks a lot

[helenzhang]

I love your video. You make things so clear. Thank you!

[RazaKhan-dsby]

Simply excellent way too much clarity in his lecture. You are Star.

[AmineSamus]

Hi this was a great lecture. Im curious though, if the peaks give the same retention time in the chromatogram for multiple analytes, how do you separate them and determine which analyte is which?

Also does elution just mean how fast it will be detected by the detector? So a polar analyte will have a lower elution time?

[kuntarinirahsilawati]

Thank you very much. Very helping to understand.

[MahmoodHashem]

thanks a lot i am waiting the coming videos.

[marcellagiovani]

Thank you so much for all these videos! it really helped me a lot in understanding and perhaps some troubleshooting on HPLC :)) maybe the next HPLC video could be somewhere around trouble shooting the result of the peaks? maybe on the shape of the peak, what is a good shape, what is a bad shape, what does it mean and what can we do to it. and maybe by then we can do some data analysis? :D thank you!!

[azizabed]

Hi,

Do you have any lectures about different types of columns?

Analytical and preparative columns?

Thanks