Basic Guide on How to Use the HPLC

Simple background knowledge on the HPLC and how to use it. Well, how I personally use it. Feel free to ask questions, this is for all the students are just starting on research!

[Volkanvarli]

You have to use vial cap with septus to avoid carryover. Especially if you use autosampler. Also applies to manual injector. Nice video.

[tristianand]

Amazing video. Does HPLC use filters at all? Or is it just to filter the mobile phase. I had a friend say their peaks looked wrong b/c they were accidentally using UPLC filters instead while running the HPLC.

[bpopa]

I love the dance at the end and cool graphics. Go Science!

[speakArabiceasilywithme]

For which type of sample It is suitable... Liquid only??

[christianlopez-ramirez]

Does this require physical contact with sample surface?

[বিন্দাস-শ৬ঘ]

What type of PC configuration is required for the Sample analysis in HPLC?

[wrongamount]

Since you're using reverse phase hplc, how does size-exclusion hplc differ from this? is it only the column used which is different??

[chitigori]

sir mujy hplc ka software chahye...

sir i need the software of hplc.. ( Hitachi )

[shivakarthakur]

Research associate
Association
Physiology

[WattRosemary-it]

Thomas Charles Rodriguez Edward Lee Donna

[PedroBaker-bd]

Perez Mark Anderson Maria Thompson Nancy

[BruceWheeler-co]

Clark George Lewis Kimberly Moore Shirley

[vitthalkabade]

P/ s,
Sir sent add, & phone number HPCL gc is traning institute in Pune or pcmc.