1) Next Generation Sequencing (NGS) - An Introduction

What is Next Generation Sequencing?
➜ Next Generation Sequencing (NGS) is a powerful platform that has enabled the sequencing of thousands to millions of DNA molecules simultaneously. This powerful tool is revolutionizing fields such as personalized medicine, genetic diseases, and clinical diagnostics by offering a high throughput option with the capability to sequence multiple individuals at the same time.

Corrections:
➜ [0:35] Sanger Sequencing was developed in 1977, not 1900s
➜ [2:22] The video refers to an "Illumina MySeq" machine - the correct name of the machine is actually "Illumina MiSeq"

For more information on Next Generation Sequencing (NGS), please visit:

For a list of other free NGS resources, visit:

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[Biomeducated]

Here's my homework summary:
0:06 General overview
0:12 What is NGS?
0:33 Sanger sequencing
2:16 NGS overview
1) 2:39 Sample preparation
2) 2:51 Sequencing machines
3) 3:11 Data output
3:23 Different NGS platforms
1) 3:40 Pyrosequencing
2) 4:33 Sequencing by synthesis
3) 5:31 Sequencing by ligation
4) 6:42 Ion semiconductor sequencing
7:19 Comparison of NGS platforms: coverage of genome per read
8:26 Applied Biological Material's NGS services

I must say, this video is probably more helpful for any Bs, Ms or PhD student than a 1h lecture by some Professor...
High educational value!
Thanks, ABM!

[timothywright]

Explains in 5 minutes what my lecturer tried to in 30 mins....straightforward, uncomplicated video.
However like similar comments read, the woman talks far too quickly and the background music is an unwanted distraction.

[shekarsubramanian]

A very comprehensive video for anyone who wants to get a brief overview on NGS, the methods and the comparison between them under 10 mins. Highly recommended.

[arnaudj]

I'm a french medical student and you've helped me a lot understanding biochemistry ! thank you !

[antoninbourdin]

I have been trying to understand the specifics of NGS for days now, and I understood it all after one watch. Thank you so much

[kareemjeiroudi]

This is a really good brief summary of the most commonly use NGSs. I watched that the night before my exam and had them all stuck in my mind

[NightPaddle]

NGS seems like a useful tool for the home kitchen. For example, if I want to know if my italian extra virgin olive oil really contains italian olives. As a student I face this problem every day, since I can't afford expensive italian brands.

[Janvanelslande]

Best video on this subject that I've seen so far

[harrisonwynn]

I'm glad this exists. This was the only simple explanation of NGS I could find.

[tomsadeh]

Great video, good explanations and perfect speaking speed! Literally got me a first class honours on a 'Compare & Contrast the NGS Platforms' final year assignment! Appreciated :)

[lbow]

we have a few of your pcr machines just for the ramp speeds. my 6-9kb kb assays always have great bands. keep it up!

[OmountainO]

This was an excellent video! I enjoyed it. I think it is worth mentioning that the helicos sequencer, one of the NGS technology, does not require amplification during the sample preparation time.

[SamSays]

this was very helpful & informative. I liked how the video notes were typed out so, instead of frantically taking notes on what was said, I could focus on the process and animations. Also, the overall diagram on the page is amazing.

[jackkennedy]

This video shall constitute a full essay for my final year exam in biotech lol

[dawnhoran]

Hi abm, thank you for an really great video which clearly explained the differences between these platforms.  It was very helpful to have the text and a clear image of the  steps involved.  Loved the animations.

[Seebzters]

Sequencing by synthesis is used by Illumina. It is step-by-step incorporation of a reversible fluorescent and a terminated nucleotide. All the bases are added to the sequencing chip. Once a base is incorporated, the rest are washed away. The fluorescent is read and recorded. The fluorescent marker and the terminated nucleotide is removed. This process is repeated until gene sequencing is complete. It eliminates the homopolymer error issue but it was has increased error due to increased read lengths.

[BiteSized]

ciao ti seguo da quando la DNA polimerasi non era ancora stata scoperta, spero che leggiate il mio commento, vi mando un abbraccio 🦝😽😽

[zhoumowei]

very nice general introduction for people who are not experts in the field

[elisebreedlove]

I didn't find her to be too fast. Besides, if you need to, you can slow down the video a tad; there's no need to get bent out of shape. Very informational video! Thank you for your time and effort.

[summerbreezenight]

I wish you did not use the background music.