Next Generation Sequencing (Illumina) - An Introduction

Hey Friends,

you wanted to know how this incredibly fast sequencing technique of the recent years works? Next Gen Sequencing includes the so called ´Sequencing by Synthesis` and with that procedure it is possible to sequence a human genome in a single day. My explanation here is based on the system developed by Illumina.

Support me here:

For your interest:

Very good animated video on how the Next Gen Seq machines of ILLUMINA work (check it out!)

Another cool video:

Have a good day!

Cheers,
Henrik

[ammaralramdhan]

Who else came here cause the illumina video was confusing

[almudenaotalora]

Incredible well explained. Non of my uni professors have been able to explained that well

[ericchen]

One thing to keep in mind is that adaptors are stuck with the 5’ end to the flow cell so the polymerase can synthesize DNA in 5’ to 3’ direction

[sonya]

all the other vids on youtube just kinda goes on a rampage abt how innovative and amazing it is like a promotion or smth. So thank u so much my guy

[bobu]

Finally somebody explaining how the technique actually works. Over videos are so vague and assume you already understand the function of all the parts. Thanks so much

[suranjanasikdar]

This is the best explanation on NGS. I had no clue, done so many researches but cant understand clearly. But this explanation is just awesome. Thank you so much.

[kairenyeong]

I wish I found this video earlier lol, spent 2 semesters on this and none of my professors explained it well enough
Now everything make sense to me

[Goldbio]

Just want to give you a shout- out and thank you for such an easy-to-understand overview of this process. It is definitely helpful.

[bertramaudiel]

A perfect and clear explanation for the NGS!

[jaysung]

thank you very much sir. This is more well explained than the official Illumina videos.

[henrikslab]

Which topics should I cover in my next videos? Make suggestions below this comment and like the ones you are most interest in

[noorkhan]

Excellent, very well explained. Clearing all the concepts one by one

[hashrafi]

simple, clear, and comprehensive! Great job!

[henrikslab]

From now on you can become a member of this channel! You will have access to some cool emojis like or .
This is of course voluntary, but the financial support helps me a lot.

[BenWinney]

Great video. The only one that allowed me to understand this difficult topic

[hifza]

What is NGS sequencing?
For that what we do first is fragment our DNA.
Each fragment will then be attached to two adapters, a side chain nucleotide binding sequence and an oligonucleotide that will bind to the flow cell.
The DNA is denatured and the fragments then attach to the flow cell. Here DNA amplification by PCR results. The unattached atrand is washed away.
Hence, bridge amplification is carried out where the strand attached via both it's ends to the flow cell and the PCR is carried out. In this way, both the strands are attached to the flow cell. After several rounds we have the amplified DNA. Having both forward and reverse strands.
The reverse strands are washed away and the forward strands undergo sequencing by synthesis
Here each strand has primer attached and one by one a fluorescently labeled nucleotide attaches to the DNA, which is detected each time a nucleotide is incorporated, once the DNA fragments are replicated and nucleotide for one particular strand is detected along with for other strands, bioinformatics tools are used to determine the sequence of entire fragments by placing the sequences side by side and determining the sequence overlaps. The sequence are compared to reference genome

[henrikslab]

Make sure to subscribe to the channel here

[amanaparbin]

Such a amazing esiyer undesirable way of teaching ❤️ we need teacher like u . Great 👍🙏

[litchfirmian]

Thank you, your animation and explanation are very clear. I finally understand what happened.

[hananghozlan]

this is incredibly clear and easy, thank you very much