NGS Data Analysis 101: RNA-Seq, WGS, and more - #ResearchersAtWork Webinar Series

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Today's webinar agenda:
1. Brief Review of Next Generation Sequencing
2. Understanding NGS Data Outputs
3. Whole Genome Sequencing Data Analysis
4. Working with RNA-Seq Data
5. Wrap Up + Q&A

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New to bioinformatics analysis for NGS data and want to learn more?

One of the benefits of Next Generation Sequencing is the large amount of data that can be generated for each sample and each project. Often, though, it can be challenging to make sense of your data and perform meaningful analysis to plan the next stages of your research.

For all types of NGS, the sequencing is often only the beginning of the project. Once you have this data, you may want to look at small insertions or deletions in your samples (Whole Genome Sequencing), learn more about differentially-expressed genes after treating your cells with a specific drug (RNA-Seq), or determining what prokaryotes compose the microbial community that is present in a soil or water sample (16S rDNA Metagenomics).

Sorting through large data sets and interpreting your results can often be a bit intimidating.

In this webinar, we'll discuss data analysis for NGS data, including running through examples for different types of analysis you may want to do for your project!

You'll learn about:
•An introduction into processing NGS data
•How that data can be used for different types of analysis, including:
- Variant Calling
- Differential Gene Expression
- and more
•Troubleshooting common issues that can arise during analysis

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I work as a bioinformatician and take a lot of these things for granted. Thank you for explaining this really well! It was still very instructive going through this video and filling the gaps in my knowledge.

arpitpanda

That's amazing. Do you guys have any tutorials or one to one hand on training for beginners?

ranjithkumar

Wonderful. Really informative, thanks for this.

Tenai-

Thank you for video. That is so informative and understandable

esmabilen

do they compare healthy cells vs cancer cells for the same organism?

Michael-ills

Very informative video for who, as me, is approaching to NGS world.

rosaloffredo

Hey can you please provide any video related to CRISPR data analysis?? After getting desired gRNA and target sequence

Its_InduB

Deseq2 needs raw counts, not normalised counts. Why are you suggesting to run Deseq2 after Stringtie normalisation? Thank your for the video

ralsg

Very helpful, this video guide me how to design experiments. But I've haven't understood about the Variant calling Analysis, please notice me if you post something related to this topic. Thank you very much.

mochipham

Thank you very much. Very imformative.

HewanDemissie

I want to analysis NGS data in linux. So how can i do that

johirislam

Hi there,
Its been a while that I am using this book to learn how to analyze Genome data: "Computational Exome and genome Analysis"

But I need a faster way to watch webinars or videos and learn how to analyze genome instead of reading this thick book!

Do you have any suggestions to me? Any channels or websites?

alisaber

can you also show us the command lines used for sequencing either in linux ???

adarshraj.j

Assalamualaikum mohon ijin bergabung kami Jalasenastri Ny.eka agus riyanto Ranting G cabang 3 KP 2 Surabaya.Terimakasih🙏

ekapurwanti

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