Enzyme Linked Immunosorbent Assay (ELISA) Operation Procedure (Sandwich Method)

Sandwich ELISA (or sandwich immunoassay) is the most commonly used elisa method. This format requires two antibodies/antigens specific for different epitopes of the antigen/antibodies. These two antibodies/antigens are normally referred to as matched antibody/antigen pairs. One of the antibodies/antigen is coated on the surface of the micro plate and used as the capture antibody/antigen to immobilize the tested analyte. The other antibody/antigen is conjugated with biotin or other markers to facilitate the detection of the analyte.

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[aakifinayat]

do we need to dilute and make different concentrations of our sample too?? just like you did it for the standard?

[aakifinayat]

how many samples did you tested? and you tested each sample in duplicates? i have 2 samples but I am confused how to prepare my plate? if someone can help me out

[walternguyen]

Sorry, can I ask a question: Why did you mix, vortex, and centrifuge the mixture containing 500ul Reference Standard & Sample Dilute from a 1ml vial (that you stand for 1-2mins) and 500ul from one of 7 tubes maintaining the same solution? Whether these solution has the same sources (from a bottle tagged name with Reference Standard & Sample Dilute)

[rajputananigam]

I want to.ask you are using dupicate wells for patient sample and same for standard?

[-_BillySaputraS]

does elisa kit comes with its reagent and its buffer?